Hot water extraction and chromatographic purification methods were used to extract and purify two polysaccharides (RAPS-1 and RAPS-2) from the roots of alfalfa. Subsequently, RAPS-2 was modified using the HNO₃/Na₂SeO₃ method to obtain Se-RAPS-2. The structural features, antioxidant and in vitro anti-tumor activities of the three polysaccharides were evaluated. The structural analysis revealed that RAPS-1 (Mw = 10.0 kDa) was composed of rhamnose, xylose, arabinose, galacturonic acid, mannose and glucose, whereas RAPS-2 (Mw = 15.8 kDa) consisted of rhamnose, xylose, galacturonic acid, mannose, glucose and galactose. RAPS-1 contained 1 → 2, 1 → 4, 1 → 3, and 1 → 6 or 1 → glycosidic bonds; however, while RAPS-2 lacked 1 → 4 glycosidic linkages. The molecular weight of Se-RAPS-2 was 11.0 kDa less than that of RAPS-2. The results of activities demonstrated that Se-RAPS-2 displayed superior antioxidant activity and inhibitory effect in HepG2 cells than RAPS-1 and RAPS-2.

使用热水提取和色谱纯化方法从苜蓿的根中提取和纯化两种多糖（RAPS-1和RAPS-2）。随后，使用HNO ₃ / Na ₂ SeO ₃修饰RAPS-2获得Se-RAPS-2的方法。评价了这三种多糖的结构特征，抗氧化剂和体外抗肿瘤活性。结构分析表明，RAPS-1（Mw = 10.0 kDa）由鼠李糖，木糖，阿拉伯糖，半乳糖醛酸，甘露糖和葡萄糖组成，而RAPS-2（Mw = 15.8 kDa）由鼠李糖，木糖，半乳糖醛酸，甘露糖组成。 ，葡萄糖和半乳糖。RAPS-1包含1→2、1→4、1→3和1→6或1→糖苷键; 然而，尽管RAPS-2缺乏1→4糖苷键。Se-RAPS-2的分子量比RAPS-2小11.0 kDa。活性结果表明，Se-RAPS-2在HepG2细胞中显示出比RAPS-1和RAPS-2更好的抗氧化活性和抑制作用。