Transcriptomic analysis of an l-threonine-producing Escherichia coli TWF001.,Biotechnology and Applied Biochemistry

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Transcriptomic analysis of an l-threonine-producing Escherichia coli TWF001.
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-01-24 , DOI: 10.1002/bab.1890
Lei Zhao _{1,

2} , Hailing Zhang ₃ , Xiaoyuan Wang _{1,

2,

4} , Guoqiang Han ₅ , Wenjian Ma _{1,

4} , Xiaoqing Hu _{1,

4} , Ye Li ₁

Affiliation

Wild‐type Escherichia coli usually does not accumulate l ‐threonine, but E. coli strain TWF001 could produce 30.35 g/L l ‐threonine after 23‐H fed‐batch fermentation. To understand the mechanism for the high yield of l ‐threonine production in TWF001, transcriptomic analyses of the TWF001 cell samples collected at the logarithmic and stationary phases were performed, using the wild‐type E. coli strain W3110 as the control. Compared with W3110, 1739 and 2361 genes were differentially transcribed in the logarithmic and stationary phases, respectively. Most genes related to the biosynthesis of l ‐threonine were significantly upregulated. Some key genes related to the NAD(P)H regeneration were upregulated. Many genes relevant to glycolysis and TCA cycle were downregulated. The key genes involved in the l ‐threonine degradation were downregulated. The gene rhtA encoding the l ‐threonine exporter was upregulated, whereas the genes sstT and tdcC encoding the l ‐threonine importer were downregulated. The upregulated genes in the glutamate pathway might form an amino‐providing loop, which is beneficial for the high yield of l ‐threonine production. Many genes encoding the 30S and 50S subunits of ribosomes were also upregulated. The findings are useful for gene engineering to increase l ‐threonine production in E. coli .

中文翻译：

产生L-苏氨酸的大肠杆菌TWF001的转录组学分析。

野生型大肠杆菌中通常不累积升-苏氨酸，但大肠杆菌菌株TWF001可能产生30.35克/ L升-苏氨酸23-H后补料分批发酵。为了理解的高产率机构升-苏氨酸生产中TWF001，进行在对数和稳定期收集的TWF001细胞样品的转录组学分析中，使用野生型大肠杆菌菌株W3110作为对照。与W3110相比，分别在对数期和固定期差异转录了1739和2361个基因。大多数与l的生物合成有关的基因苏氨酸显着上调。与NAD（P）H再生有关的一些关键基因被上调。与糖酵解和TCA循环有关的许多基因被下调。参与的关键基因升苏氨酸降解的下调。基因rhtA基因编码升-苏氨酸出口被上调，而基因SSTT和TDCC编码升-苏氨酸进口商被下调。谷氨酸途径中的上调基因可能会形成一个氨基提供环，这有利于l的高产苏氨酸生产。编码核糖体的30S和50S亚基的许多基因也被上调。这些发现可用于基因工程来增加升-苏氨酸生产大肠杆菌。

更新日期：2020-01-24

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