当前位置:
X-MOL 学术
›
Chem. Eur. J.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A Quadrupolar Bis‐Triarylborane Chromophore as a Fluorimetric and Chirooptic Probe for Simultaneous and Selective Sensing of DNA, RNA and Proteins
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2020-01-24 , DOI: 10.1002/chem.201905567 Željka Ban 1 , Stefanie Griesbeck 2 , Sanja Tomić 1 , Jörn Nitsch 2 , Todd B. Marder 2 , Ivo Piantanida 1
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2020-01-24 , DOI: 10.1002/chem.201905567 Željka Ban 1 , Stefanie Griesbeck 2 , Sanja Tomić 1 , Jörn Nitsch 2 , Todd B. Marder 2 , Ivo Piantanida 1
Affiliation
One dye to detect them all: A tetracationic quadrupolar bis‐triarylborane dye can bind with 10–100 nM affinity and efficiently distinguish between DNA/RNA and BSA by characteristic fluorescence emission points separated by Δ
=3600 cm−1, allowing the simultaneous quantification of DNA/RNA and protein (BSA) in a mixture. The applicability of such fluorimetric differentiation in vitro was demonstrated, strongly supporting a protein‐like target as a dominant binding site of this dye in human cells. More information can be found in the Full Paper by T. B. Marder, I. Piantanida, et al. (DOI: 10.1002/chem.201903936).
中文翻译:
四极双-三芳基硼烷发色团,用于同时,选择性感应DNA,RNA和蛋白质的荧光和灵敏探针。
一种可以全部检测的染料:四阳离子四极双三芳基硼烷染料可以10–100 nM的亲和力结合,并通过以Δ = 3600 cm -1分隔的特征性荧光发射点有效区分DNA / RNA和BSA ,从而可以同时定量混合的DNA / RNA和蛋白质(BSA)。此类荧光分光光度法在体外的适用性得到了证明,强烈支持了蛋白质样靶标作为该染料在人类细胞中的主要结合位点。可以在T. B. Marder,I. Piantanida等人的论文全文中找到更多信息。(DOI:10.1002 / chem.201903936)。
更新日期:2020-01-24
中文翻译:
四极双-三芳基硼烷发色团,用于同时,选择性感应DNA,RNA和蛋白质的荧光和灵敏探针。
一种可以全部检测的染料:四阳离子四极双三芳基硼烷染料可以10–100 nM的亲和力结合,并通过以Δ = 3600 cm -1分隔的特征性荧光发射点有效区分DNA / RNA和BSA ,从而可以同时定量混合的DNA / RNA和蛋白质(BSA)。此类荧光分光光度法在体外的适用性得到了证明,强烈支持了蛋白质样靶标作为该染料在人类细胞中的主要结合位点。可以在T. B. Marder,I. Piantanida等人的论文全文中找到更多信息。(DOI:10.1002 / chem.201903936)。