17β-estradiol (E2) often coexists with tetracyclines (TCs) in wastewater lagoons at intensive breeding farms, threatening the quality of surrounding water bodies. Microbial degradation is vital in E2 removal, but it is unclear how TCs affect E2 biodegradation. This primary study investigated the mechanisms of E2 degradation by Novosphingobium sp. ES2-1 in the presence of TCs and assessed the removal efficiency of E2 by strain ES2-1 in natural waters containing TCs. E2 biodegradation was unaffected at TCs concentrations below 0.1 mg L⁻¹ yet significantly inhibited at TCs above 10 mg L⁻¹. As elevation of TCs, E2 biodegradation rate constant decreased, and the biodegradation kinetics equation gradually deviated from the pseudo-first-order dynamics model. Importantly, the presence of TCs, especially at high-level concentrations, significantly hindered E2 ring-opening process but promoted the condensation of some phenolic ring-opening products with NH₃, thereby increasing the abundance of pyridine derivatives, which were difficult to decompose over time. Additionally, strain ES2-1 could remove 52.1–100% of nature estrogens in TCs-contaminated natural waters within 7 d. Results revealed the mechanisms of TCs in E2 biodegradation and the performance of a functional strain in estrogen removal in realistic TCs-contaminated aqueous solution.

17 β雌二醇（E2）经常与废水泻湖四环素（TCS）在集约化养殖场，威胁周围水体的质量并存。微生物降解对于E2的去除至关重要，但目前尚不清楚TC如何影响E2的生物降解。这项主要研究调查了新孢子菌对E2降解的机理。ES2-1在存在TC的情况下，评估了菌株ES2-1在含有TC的天然水中对E2的去除效率。E2生物降解在TCs浓度低于0.1 mg L ^-1时不受影响，但在TCs高于10 mg L ^-1时显着抑制。随着TCs的升高，E2生物降解速率常数降低，生物降解动力学方程逐渐偏离拟一级动力学模型。重要的是，TC的存在（特别是在高浓度下）显着阻碍了E2开环过程，但促进了某些酚类开环产物与NH ₃的缩合，从而增加了吡啶衍生物的丰度，这些吡啶衍生物很难分解。时间。此外，菌株ES2-1可以在7天之内去除受TCs污染的天然水中52.1–100％的自然雌激素。结果揭示了TCs在E2生物降解中的机制以及功能菌株在实际的TCs污染水溶液中去除雌激素的性能。