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Metagenomic analysis reveals enhanced nutrients removal from low C/N municipal wastewater in a pilot-scale modified AAO system coupling electrolysis.
Water Research ( IF 11.4 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.watres.2020.115530 Wei Huang 1 , Benzhou Gong 1 , Yingmu Wang 1 , Ziyuan Lin 1 , Lei He 1 , Jian Zhou 1 , Qiang He 1
Water Research ( IF 11.4 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.watres.2020.115530 Wei Huang 1 , Benzhou Gong 1 , Yingmu Wang 1 , Ziyuan Lin 1 , Lei He 1 , Jian Zhou 1 , Qiang He 1
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The conventional biological nutrients removal process is challenged by insufficient organic carbon in influent. To cross such an organic-dependent barrier, a pilot-scale electrolysis-integrated anaerobic/anoxic/oxic (AAO) process was developed for enhanced removal of nitrogen (N) and phosphorus (P) from low carbon/nitrogen (C/N) municipal wastewater. Average removal efficiencies of total nitrogen (TN) and total phosphorus (TP) in the electrolysis-AAO reached to 77.24% and 95.08% respectively, showing increases of 13.88% and 21.87%, as compared to the control reactor. Spatial variations of N and P showed that NH4+-N removal rate was promoted in aerobic zone of electrolysis-AAO. The intensified TN elimination, which was mostly reflected by abatement of NO3--N with the concomitant slight accumulation of NH4+-N and NO2--N, mainly occurred in anoxic2 compartment as the electrons supplied by electrolysis. Furthermore, minor P contents were measured and remained almost unchanged along the reaction units, indicating that chemical precipitation should be the dominant mechanism of P-removal in electrolysis-AAO. From the metagenomic-based taxonomy, phylum Actinobacteria was dramatically inhibited, and phylum Proteobacteria dominated the electrolysis-AAO. Particularly, nitrifying bacteria and multifarious autotrophic denitrifiers were enriched, meanwhile, a significant evolution of heterotrophic denitrifiers was found in electrolysis-AAO compared to control, which was mostly reflected by the inhibition of genus Candidatus Microthrix. Batch tests further confirmed that autotrophic denitrifiers using H2 and Fe2+ as essential electron sinks were mainly responsible for the electrolysis-induced denitrification. Differential metabolic capacities were revealed from the perspectives of functional enzymes and genes, and network analysis allowed insight of microbial taxa-functional genes associations and shed light on stronger relevance between autotrophic denitrifiers and denitrification-associated genes in the electrolysis-AAO system.
中文翻译：
元基因组学分析显示，在耦合电解的中试规模改进AAO系统中，从低C / N市政废水中去除的营养物得到了增强。
常规的生物营养去除过程受到进水有机碳不足的挑战。为了克服这种依赖于有机物的障碍，开发了一种中试规模的电解厌氧/缺氧/有氧（AAO）工艺，以增强从低碳/氮（C / N）中去除氮（N）和磷（P）的能力。市政废水。电解AAO中总氮（TN）和总磷（TP）的平均去除率分别达到77.24％和95.08％，与对照反应器相比，分别提高了13.88％和21.87％。N和P的空间变化表明，在电解AAO的好氧区中，NH4 + -N的去除率提高。强化消除总氮，主要反映为减少了NO3--N并伴有少量NH4 + -N和NO2--N的积累，主要发生在缺氧隔室中，是由电解提供的电子。此外，测定了微量的P含量，并沿反应单元几乎保持不变，表明化学沉淀应是电解AAO中P去除的主要机理。从基于宏基因组学的分类学中，放线菌门菌被显着抑制，而Proteobacteria菌在电解AAO中占主导地位。尤其是，硝化细菌和多种自养反硝化菌都富集了，与此同时，与对照相比，电解-AAO中发现了异养反硝化菌的显着进化，这主要是通过抑制假丝酵母菌而得到的。分批测试进一步证实，使用H2和Fe2 +作为必需电子吸收体的自养反硝化剂主要负责电解诱导的反硝化作用。从功能性酶和基因的角度揭示了不同的代谢能力，并且网络分析可以洞察微生物的分类单元功能基因的关联，并揭示了电解AAO系统中自养反硝化剂与反硝化相关基因之间更强的相关性。
更新日期：2020-01-23
中文翻译：
元基因组学分析显示，在耦合电解的中试规模改进AAO系统中，从低C / N市政废水中去除的营养物得到了增强。
常规的生物营养去除过程受到进水有机碳不足的挑战。为了克服这种依赖于有机物的障碍，开发了一种中试规模的电解厌氧/缺氧/有氧（AAO）工艺，以增强从低碳/氮（C / N）中去除氮（N）和磷（P）的能力。市政废水。电解AAO中总氮（TN）和总磷（TP）的平均去除率分别达到77.24％和95.08％，与对照反应器相比，分别提高了13.88％和21.87％。N和P的空间变化表明，在电解AAO的好氧区中，NH4 + -N的去除率提高。强化消除总氮，主要反映为减少了NO3--N并伴有少量NH4 + -N和NO2--N的积累，主要发生在缺氧隔室中，是由电解提供的电子。此外，测定了微量的P含量，并沿反应单元几乎保持不变，表明化学沉淀应是电解AAO中P去除的主要机理。从基于宏基因组学的分类学中，放线菌门菌被显着抑制，而Proteobacteria菌在电解AAO中占主导地位。尤其是，硝化细菌和多种自养反硝化菌都富集了，与此同时，与对照相比，电解-AAO中发现了异养反硝化菌的显着进化，这主要是通过抑制假丝酵母菌而得到的。分批测试进一步证实，使用H2和Fe2 +作为必需电子吸收体的自养反硝化剂主要负责电解诱导的反硝化作用。从功能性酶和基因的角度揭示了不同的代谢能力，并且网络分析可以洞察微生物的分类单元功能基因的关联，并揭示了电解AAO系统中自养反硝化剂与反硝化相关基因之间更强的相关性。
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