The anticancerous effects of PCHPs (HBSS, CHSS, DASS, and CASS) were investigated on Human cervical cancer Hela cells proliferation inhibition, cytotoxicity, caspase-3 activity, cell cycle, and apoptosis. The inhibition rate was expressed as CASS > HBSS > CHSS > DASS, with the maximum inhibition of 74.453 ± 3.399%. Cell cytotoxicity was observed (CASS > CHSS > HBSS > DASS) with the maximum cell death rate of 82.472 ± 3.488%. The caspase-3 activity was induced by CASS > HBSS > DASS > CHSS, with the maximum multiple of 2.954 ± 0.103. CASS induced cell cycle block at the G2/M phase by elevating mRNA expression of CyclinD1, p21, p53 and Wee1, and lowering the expression of Survivin, CHK2, Wee1, CyclinB1, and CDK-1. CASS enhanced the mRNA expression of DR3, DR5, FasL, FADD, PARP, TNF- α, TNF- R1, TRDAA, caspases-8, caspases-10 and the protein expression of FasL and caspases-8, -10 in the death receptor pathway; while, lowered the mRNA expression of antiapoptotic genes (Bcl - 2 and Bcl-xL) and the protein expression of Bcl - 2. The mRNA expression of apoptosis genes (Bak, Cytc, Puma, and caspases-3, -7, -9) and the protein expression of caspases-3, -9 of mitochondria pathway was up regulated which led to cell apoptosis.

中文翻译：

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从人参中提取的多糖对人宫颈癌Hela细胞的抗癌作用。

研究了PCHP（HBSS，CHSS，DASS和CASS）对人宫颈癌Hela细胞增殖抑制，细胞毒性，caspase-3活性，细胞周期和凋亡的抗癌作用。抑制率表示为CASS> HBSS> CHSS> DASS，最大抑制率为74.453±3.399％。观察到细胞毒性（CASS> CHSS> HBSS> DASS），最大细胞死亡率为82.472±3.488％。caspase-3活性由CASS> HBSS> DASS> CHSS诱导，最大倍数为2.954±0.103。CASS通过提高CyclinD1，p21，p53和Wee1的mRNA表达，并降低Survivin，CHK2，Wee1，CyclinB1和CDK-1的表达来诱导G2 / M期细胞周期阻滞。CASS增强了DR3，DR5，FasL，FADD，PARP，TNF-α，TNF-R1，TRDAA，caspases-8的mRNA表达，死亡受体途径中的caspases-10和FasL和caspases-8，-10的蛋白表达；同时降低了抗凋亡基因（Bcl-2和Bcl-xL）的mRNA表达以及Bcl-2的蛋白表达。凋亡基因（Bak，Cytc，Puma和caspases-3，-7，-9）的mRNA表达降低了。 ），线粒体途径的caspases-3，-9的蛋白表达上调，导致细胞凋亡。