Timely antibacterial treatment of wounds reduces the probability of wound infection and promotes wound healing. However, the materials used to treat wounds often fail to provide both sterilization (especially for super bacteria) and moisture, and some may even cause secondary injury to the wound. In this study, gold nanoparticles (Au NPs) of average grain diameter of 3 ± 1 nm were prepared using egg white as the reductant. These particles showed no aggregation and pink fluorescence. Au NPs were mixed with gentamicin sulfate (GS) and loaded into a mixture of konjac glucomannan (KGM) and gelatin as wound dressing (KGM/Gelatin@Au NPs/GS). Antibacterial experiments showed that the Au NPs amplified the antibacterial activity of GS; Au NPs/GS efficiently eliminated bacteria, especially super bacteria. Cytotoxicity tests indicated that KGM/Gelatin@Au NPs/GS showed basically no cytotoxicity to L929 cells. In addition, KGM/Gelatin@Au NPs/GS possesses good water absorption, water retention, and enhanced mechanical properties, which can provide a moist environment for wounds and promote healing. In conclusion, our study showed that the antibacterial activity of KGM/Gelatin@Au NPs/GS is better than that of only GS and that it efficiently eliminated super bacteria. Therefore, KGM/Gelatin@Au NPs/GS can be used for killing superbugs, inhibiting bacterial growth, and promoting wound healing.

中文翻译：

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蛋白质还原的金纳米颗粒与硫酸庆大霉素混合并装入魔芋/明胶海绵中，可以治愈伤口并杀死耐药细菌。

及时的伤口抗菌处理可降低伤口感染的可能性并促进伤口愈合。但是，用于治疗伤口的材料通常无法同时提供灭菌作用（尤其是对超级细菌而言）和水分，有些甚至可能对伤口造成二次伤害。在这项研究中，使用蛋清作为还原剂制备了平均粒径为3±1 nm的金纳米颗粒（Au NPs）。这些颗粒未显示聚集和粉红色荧光。将金纳米颗粒与硫酸庆大霉素混合，并装入魔芋葡甘露聚糖（KGM）和明胶的混合物中作为伤口敷料（KGM / Gelatin @ Au NPs / GS）。抗菌实验表明，金纳米颗粒增强了GS的抗菌活性。Au NPs / GS有效消除细菌，尤其是超级细菌。细胞毒性试验表明，KGM / Gelatin @ Au NPs / GS对L929细胞基本无细胞毒性。此外，KGM / Gelatin @ Au NPs / GS具有良好的吸水性，保水性和增强的机械性能，可以为伤口提供潮湿的环境并促进愈合。总之，我们的研究表明，KGM / Gelatin @ Au NPs / GS的抗菌活性优于仅GS，并且能有效消除超级细菌。因此，KGM / Gelatin @ Au NPs / GS可用于杀死超级细菌，抑制细菌生长并促进伤口愈合。我们的研究表明，KGM / Gelatin @ Au NPs / GS的抗菌活性优于仅GS，并且能有效消除超级细菌。因此，KGM / Gelatin @ Au NPs / GS可用于杀死超级细菌，抑制细菌生长并促进伤口愈合。我们的研究表明，KGM / Gelatin @ Au NPs / GS的抗菌活性优于仅GS，并且能有效消除超级细菌。因此，KGM / Gelatin @ Au NPs / GS可用于杀死超级细菌，抑制细菌生长并促进伤口愈合。