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Visible light excited ratiometric-GECIs for long-term in-cellulo monitoring of calcium signals.
Cell Calcium ( IF 4.3 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.ceca.2020.102165 Jia Li 1 , Liuqing Wang 1 , Yilan Chen 1 , Yang Yang 2 , Jin Liu 1 , Kai Liu 1 , Yi-Tsang Lee 3 , Ningjia He 4 , Yubin Zhou 3 , Youjun Wang 5
Cell Calcium ( IF 4.3 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.ceca.2020.102165 Jia Li 1 , Liuqing Wang 1 , Yilan Chen 1 , Yang Yang 2 , Jin Liu 1 , Kai Liu 1 , Yi-Tsang Lee 3 , Ningjia He 4 , Yubin Zhou 3 , Youjun Wang 5
Affiliation
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Genetically Encoded Calcium Indicators (GECIs) are powerful molecular tools for monitoring calcium (Ca2+) signaling in the cytosol and organellar compartments. However, currently available ratiometric indicators that allow measurements of resting Ca2+ levels have limitations in long-term Ca2+ imaging. They either are ultraviolet (UV)-excited ones with strong photo-toxicity, or have poor performance. To overcome this hurdle, we developed a set of visible light excited ratiometric-GECIs (VR-GECIs) based on existing mono-colored GECIs. With performance comparable to their corresponding mono-color prototypes, this set of VR-GECIs enables long-term measurements of intra-cellular or intra-organellar Ca2+ signals. Using these VR-GECIs together with a newly developed off-line analysis tool, we achieved long-term measurements of Ca2+ homeostasis of moving or dividing cells. Our tools may find broad applications in decoding Ca2+-modulated physiological or pathological processes.
中文翻译：
可见光激发比率式-GECI用于长期细胞内钙信号监测。
基因编码钙指示剂（GECI）是监测细胞质和细胞器区室中钙（Ca2 +）信号传导的强大分子工具。但是，目前可用的比例指示器可以测量静止的Ca2 +水平，但在长期的Ca2 +成像中存在局限性。它们是具有强光毒性的紫外线（UV）激发光源，或性能较差。为了克服这一障碍，我们基于现有的单色GECI开发了一套可见光激发比率式GECI（VR-GECI）。这套VR-GECI的性能可与其相应的单色原型相媲美，从而能够长期测量细胞内或细胞内Ca2 +信号。结合使用这些VR-GECI和新开发的离线分析工具，我们实现了对移动或分裂细胞的Ca2 +稳态的长期测量。我们的工具可能会在解码Ca2 +调节的生理或病理过程中找到广泛的应用。
更新日期：2020-01-23
中文翻译：
可见光激发比率式-GECI用于长期细胞内钙信号监测。
基因编码钙指示剂（GECI）是监测细胞质和细胞器区室中钙（Ca2 +）信号传导的强大分子工具。但是，目前可用的比例指示器可以测量静止的Ca2 +水平，但在长期的Ca2 +成像中存在局限性。它们是具有强光毒性的紫外线（UV）激发光源，或性能较差。为了克服这一障碍，我们基于现有的单色GECI开发了一套可见光激发比率式GECI（VR-GECI）。这套VR-GECI的性能可与其相应的单色原型相媲美，从而能够长期测量细胞内或细胞内Ca2 +信号。结合使用这些VR-GECI和新开发的离线分析工具，我们实现了对移动或分裂细胞的Ca2 +稳态的长期测量。我们的工具可能会在解码Ca2 +调节的生理或病理过程中找到广泛的应用。
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