Simultaneous detection technology has become a hot topic in analytical chemistry; however, very few reports on how to simultaneously detect small molecular contaminants and microorganisms have been in place. Aflatoxins are a group of highly toxic and carcinogenic compounds, which are produced mainly by Aspergillus flavus and Aspergillus parasiticus from section Flavi responsible for aflatoxin accumulation in stored cereals. Both aflatoxins and Aspergillus section Flavi were used to demonstrate the duplex real-time RCR method of simultaneously detecting small molecular contaminants and microorganisms. The detection of aflatoxins and Aspergillus section Flavi was carried out depending on the anti-idiotypic nanobody-phage V2-5 and aflatoxin-synthesis related gene nor-1 (=aflD), respectively. The quantitative standard curves for simultaneous detection of aflatoxins and Aspergillus section Flavi were constructed, with detection limits of 0.02 ng/ml and 8 × 102 spores/g, respectively. Naturally contaminated maize samples (n = 25) were analyzed for a further validation. The results were in good agreement between the new developed method and the referential methods (high-performance liquid chromatography and the conventional plating counts).

中文翻译：

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基于纳米抗体-噬菌体可以同时检测小分子污染物和微生物：使用致癌物黄曲霉毒素及其主要真菌曲霉属黄酮菌。在玉米储藏示范中。

同时检测技术已经成为分析化学领域的热门话题。但是，关于如何同时检测小分子污染物和微生物的报道很少。黄曲霉毒素是一组剧毒和致癌的化合物，主要由黄曲霉和副产黄曲霉产生，这些黄曲霉毒素负责黄曲霉毒素在谷物中的积累。黄曲霉毒素和曲霉属Flavi均用于证明同时检测小分子污染物和微生物的双重实时RCR方法。黄曲霉毒素和黄曲霉切片的检测分别取决于抗独特型纳米抗体噬菌体V2-5和黄曲霉毒素合成相关基因nor-1（= aflD）。建立了同时检测黄曲霉毒素和黄曲霉切片的定量标准曲线，检测限分别为0.02 ng / ml和8×102孢子/ g。分析了自然污染的玉米样品（n = 25）以进行进一步的验证。结果与新开发的方法和参考方法（高效液相色谱法和常规的镀层计​​数）完全吻合。