The aim of this study was the assessment of the effect of time exposure, temperature, distance, and organic contaminants on radiant catalytic ionization (RCI) microbicidal effectiveness. The number of all examined bacteria decreased together with time exposure of RCI. The lowest recovery was obtained, both from the rubber surface (6.36 log CFU × cm-2) and steel (6.04 log CFU × cm-2) in the case of Escherichia coli O157:H7. On the other hand, Staphylococcus aureus was isolated in the largest number (rubber: 7.88 log CFU × cm-2, steel: 7.79 log CFU × cm-2). Among the tested environmental conditions, the greatest bacterial population was re-isolated at 4°C (distance: 0.5 m, time: 24 h), whereas the lowest population was found at a distance of 0.5 m (temperature: 20°C, time: 24 h) and on surfaces without contamination. In the samples treated with RCI, the bacterial population was the lowest on non-contaminated surfaces, ranging from 3.76 log CFU × cm-2 (E. coli O157:H7) to 5.58 log CFU × cm-2 (S. aureus) for the rubber, and from 3.26 log CFU × cm-2 (E. coli O157:H7) to 5.20 log CFU × cm-2 (S. aureus) for the stainless steel. The highest bacteria number was isolated from surfaces contaminated with meat and fish pulp. The lowest bacterial reduction caused by RCI was found in the case of rubber contaminated with meat-fish pulp (24 h, 0.5 m, 20°C). The reduction rate was equal to 0.89 log CFU × cm-2 for S. aureus, 1.17 log CFU × cm-2 for Listeria monocytogenes, 1.43 log CFU × cm-2 for Salmonella Enteritidis and 1.61 log CFU × cm-2 for E. coli O157:H7. In turn, the greatest bacterial reduction was found in the case of non-contaminated steel (24 h, 0.5 m, 37°C). The reduction rate was equal to 4.52 log CFU × cm-2 for L. monocytogenes, 3.61 log CFU × cm-2 for S. Enteritidis, 2.98 log CFU × cm-2 for E. coli O157:H7 and 2.77 log CFU × cm-2 for S. aureus. RCI allows the inactivation of pathogens from stainless steel and rubber surfaces. Its efficacy is species-dependent and affected by environmental factors.

中文翻译：

---

所选环境因素对辐射催化电离杀菌效果的影响。

这项研究的目的是评估时间暴露，温度，距离和有机污染物对辐射催化电离（RCI）杀微生物效力的影响。所有检查细菌的数量随着RCI的暴露时间而减少。在大肠杆菌O157：H7的情况下，橡胶表面（6.36 log CFU×cm-2）和钢（6.04 log CFU×cm-2）的回收率最低。另一方面，分离出的金黄色葡萄球菌数量最多（橡胶：7.88 log CFU×cm-2，钢：7.79 log CFU×cm-2）。在测试的环境条件中，最大的细菌种群在4°C下重新分离（距离：0.5 m，时间：24 h），而最低的细菌种群在0.5 m的距离上（温度：20°C，时间）被分离。 ：24 h）且表面无污染。在用RCI处理的样品中，细菌种群在未受污染的表面上最低，从橡胶的3.76 log CFU×cm-2（E. coli O157：H7）到橡胶的5.58 log CFU×cm-2（金黄色葡萄球菌），从3.26 log不锈钢的CFU×cm-2（大肠杆菌O157：H7）至5.20 log CFU×cm-2（金黄色葡萄球菌）。从被肉和鱼肉污染的表面分离出最高细菌数。在橡胶被肉鱼肉污染的情况下（24 h，0.5 m，20°C），由RCI引起的细菌减少最少。金黄色葡萄球菌的减少率等于0.89 log CFU×cm-2，单核细胞增生性李斯特氏菌的减少率等于1.17 log CFU×cm-2，肠炎沙门氏菌为1.43 log CFU×cm-2，大肠杆菌为1.61 log CFU×cm-2。大肠杆菌O157：H7。反过来，在未受污染的钢中（24 h，0.5 m，37°C），细菌的减少最大。减少率等于4。单核细胞增生李斯特菌52 log CFU×cm-2，肠炎沙门氏菌3.61 log CFU×cm-2，大肠杆菌O157：H7为2.98 log CFU×cm-2，金黄色葡萄球菌为2.77 log CFU×cm-2 。RCI使不锈钢和橡胶表面的病原体失活。它的功效取决于物种，并受环境因素影响。