Poultry is a major reservoir for the pathogen Campylobacter jejuni. C. jejuni inhabits the poultry gastrointestinal tract as a part of the gut microbiota. The objective of this study was to evaluate both the survival of C. jejuni and the changes in the population dynamics of the cecal microbiome during an in vitro C. jejuni inoculation in the presence or absence of the functional metabolites of Diamond V Original XPCTM (XPC). Two independent trials were conducted. Broiler chickens (n = 6 per Trial 1 and n = 3 per Trial 2) were raised according to standard industry guidelines and euthanized on Day 41. The ceca were collected aseptically, their contents removed independently and then used in an in vitro microaerobic model with 0.1% cecal contents + Campylobacter with or without 1% XPC (w/v). Before the inoculation with a chloramphenicol resistant marker strain of C. jejuni, the cecal contents were pre-incubated with XPC at 42°C for 24 h, in a shaking incubator (200 rpm) under microaerobic conditions, then experimentally inoculated with 108/ml of C. jejuni into the appropriate treatment groups. At 0 and 24 h for Trial 1, and 48 h for Trial 2, sub-samples of the culture (n = 3 ceca, two technical replicates per ceca, XPC alone or ceca culture alone) were enumerated using a Petroff-Hausser counter, and the DNA was extracted for microbiome analysis. DNA was isolated using the Qiagen QIAamp Fast Stool DNA Mini Kit and sequenced using the Illumina MiSeq platform. The reads were filtered, normalized, and assigned taxonomical identities using the QIIME2 pipeline. The relative microbiota populations were identified via ANCOM. Altogether, evidence suggests that XPC alters the microbiome, and in turn reduces Campylobacter survival.

中文翻译：

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体外家禽盲肠培养模型的初步开发，以评估原始XPCTM减少空肠弯曲杆菌的作用及其对微生物群的潜在影响。

家禽是空肠弯曲菌的主要贮藏库。空肠弯曲杆菌作为肠道菌群的一部分栖息在禽类胃肠道中。这项研究的目的是评估在存在或不存在Diamond V Original XPCTM（XPC）功能代谢产物的情况下，体外空肠弯曲杆菌接种过程中空肠弯曲杆菌的存活率和盲肠微生物组种群动态的变化。 ）。进行了两次独立试验。根据标准行业准则饲养肉鸡（每试验1次，n = 6，第2次试验中，n = 3），并在第41天实施安乐死。 0.1％盲肠含量+弯曲杆菌，含或不含1％XPC（w / v）。在用空肠弯曲杆菌的耐氯霉素标记菌株接种之前，将盲肠内容物在微有氧条件下于摇动培养箱（200 rpm）中于42°C与XPC预孵育24 h，然后实验接种108 / ml将空肠弯曲杆菌分为适当的治疗组。在试验1的0和24小时，以及试验2的48小时，使用彼得罗夫-豪瑟（Petroff-Hausser）计数器对培养物的子样本（n = 3盲肠，每个盲肠进行两次技术重复，单独使用XPC或单独进行盲肠培养），提取DNA进行微生物组分析。使用Qiagen QIAamp Fast Stool DNA Mini Kit分离DNA，并使用Illumina MiSeq平台进行测序。使用QIIME2管道对读数进行过滤，归一化和分配分类学身份。相对微生物群通过ANCOM确定。共，