In contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in Streptomyces coelicolor. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 represses pepck at the post-transcriptional level and thus modulates the intracellular level of phosphoenolpyruvate (PEP). The expression of scr5239 in turn is dependent on the global transcriptional regulator DasR, thus creating a feedback loop regulation of the central carbon metabolism. By post-transcriptional regulation of PEPCK and in all likelihood other targets, scr5239 adds an additional layer to the DasR regulatory network and provides a tool to control the metabolism dependent on the available carbon source.

中文翻译：

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sRNA scr5239参与了天蓝色链霉菌中央代谢的反馈环调控。

与转录调控相反，对链霉菌中的转录后调控和小的非编码RNA（sRNA）的作用尚未得到很好的研究。在这里，我们专注于天蓝色链霉菌中高度保守的sRNA scr5239。蛋白质组学方法表明，sRNA调节几种代谢酶，其中包括磷酸烯醇丙酮酸羧激酶（PEPCK），这是中央碳代谢的关键酶。sRNA scr5239在转录后水平上抑制pepck，从而调节磷酸烯醇式丙酮酸（PEP）的细胞内水平。反过来，scr5239的表达取决于全局转录调节因子DasR，从而创建了中央碳代谢的反馈环调节。通过PEPCK的转录后调控以及可能的其他目标，