Soft rot caused by numerous species of Pectobacterium and Dickeya is a serious threat to the world production of potatoes. The application of bacteriophages to combat bacterial infections in medicine, agriculture, and the food industry requires the selection of comprehensively studied lytic phages and the knowledge of their infection mechanism for more rational composition of therapeutic cocktails. We present the study of two bacteriophages, infective for the Pectobacterium brasiliense strain F152. Podoviridae PP99 is a representative of the genus Zindervirus, and Myoviridae PP101 belongs to the still unclassified genomic group. The structure of O-polysaccharide of F152 was established by sugar analysis and 1D and 2D NMR spectroscopy: → 4)-α-D-Manp6Ac-(1→ 2)-α-D-Manp-(1→ 3)-β-D-Galp-(1→ 3 ↑ 1 α -l- 6 dTal p Ac 0 - 2 The recombinant tail spike protein of phage PP99, gp55, was shown to deacetylate the side chain talose residue of bacterial O-polysaccharide, thus providing the selective attachment of the phage to the cell surface. Both phages demonstrate lytic behavior, thus being prospective for therapeutic purposes.

中文翻译：

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形态不同的巴西芽孢杆菌噬菌体PP99和PP101：噬菌体PP99的尾突蛋白使O-多糖脱乙酰化并伴随感染。

由许多种类的油杆菌和迪卡氏菌引起的软腐病严重威胁着世界马铃薯的生产。噬菌体在医学，农业和食品工业中用于抵抗细菌感染的应用要求选择经过全面研究的裂解噬菌体，并需要了解其感染机理，才能更合理地配制治疗性鸡尾酒。我们目前研究两种细菌噬菌体，对巴西芽孢杆菌F152有感染力。Podoviridae PP99是Zindervirus属的代表，而Myoviridae PP101属于仍未分类的基因组。F152的O-多糖的结构通过糖分析以及1D和2D NMR光谱确定：