Abstract

Triclosan (TCS) was quantitatively analyzed for the first time in chicken samples using immunoaffinity cleanup followed by indirect competitive enzyme-linked immunosorbent assay (icELISA). Monoclonal antibodies (mAbs) against TCS were prepared. The mAb 6E1 showed high sensitivity and specificity in phosphate-buffered saline buffer, with a half maximal inhibition concentration value of 1.77 ng mL⁻¹ and a limit of detection of 0.09 ng mL⁻¹. The average recovery in spiked chicken muscle samples was 84.4–114.8%, with a relative standard deviation below 8.1%. To validate the developed icELISA method, samples spiked with different levels of TCS were analyzed by icELISA and ultra-high-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS). The consistency of results detected by icELISA and UPLC–MS/MS demonstrated that immunoaffinity cleanup followed by icELISA could also be used for other food matrices.

中文翻译：

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新型基于抗体的间接竞争性ELISA（免疫亲和纯化）的开发，用于检测鸡中的三氯生

摘要

首次使用免疫亲和纯化对鸡样品中的三氯生（TCS）进行了定量分析，随后进行了间接竞争性酶联免疫吸附测定（icELISA）。制备了针对TCS的单克隆抗体（mAb）。mAb 6E1在磷酸盐缓冲液中显示出高灵敏度和特异性，最大抑制浓度值的一半为1.77 ng mL ^-1，检测极限为0.09 ng mL ^-1。加标的鸡肌肉样品的平均回收率为84.4–114.8％，相对标准偏差低于8.1％。为了验证已开发的icELISA方法，通过icELISA和超高效液相色谱-串联质谱（UPLC-MS / MS）分析了加标有不同TCS含量的样品。icELISA和UPLC-MS / MS检测结果的一致性表明，免疫亲和纯化以及icELISA还可用于其他食品基质。