Abstract

In this work, a simple and rapid SPE–GC–FID method for simultaneous determination of free tocopherols and phytosterols in vegetable oils was developed. The use of this SPE approach for the extraction of naturally occurring tocopherols and phytosterols provides advantages such as simplicity, high speed, and ease of use. The whole method was validated, and the limits of detection of tocopherols and phytosterols were 0.05 and 0.03 mg/100 g of oil, respectively. The precisions for the determination of tocopherols and phytosterols in vegetable oils were < 10.1% in relative standard deviations. The recoveries of selected tocopherols and phytosterols spiked at low, middle, and high concentrations ranged from 83.4 to 97.7%. The developed approach was successfully applied to the analysis of free tocopherols and phytosterols in rapeseed, sunflower seed, linseed, sesame, and maize oils.

中文翻译：

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改进的SPE-GC-FID方法同时测定植物油中的游离植物甾醇和生育酚

摘要

在这项工作中，开发了一种简单而快速的SPE-GC-FID方法，用于同时测定植物油中的游离生育酚和植物甾醇。使用这种SPE方法提取天然存在的生育酚和植物甾醇可提供诸如简便，高速和易于使用的优点。验证了整个方法的有效性，生育酚和植物甾醇的检出限分别为0.05和0.03 mg / 100 g油。用于测定植物油中生育酚和植物甾醇的精密度的相对标准偏差为<10.1％。在低，中和高浓度下加标的生育酚和植物甾醇的回收率在83.4％至97.7％之间。所开发的方法已成功地应用于分析油菜籽，葵花籽，大豆籽中的游离生育酚和植物甾醇，