European Radiology Experimental ( IF 3.7 ) Pub Date :
Abstract
Background
To examine in vitro acoustic property of nonalcoholic fatty disease in mouse and human liver to identify nonalcoholic steatohepatitis (NASH).
Methods
The acoustic impedance (× 106 kg/m2/s) was measured in 35 free fatty acids (FFAs, 500 mmol/L) and histologically-diagnosed liver samples of twelve mice (four control, four simple steatosis [SS], and four NASH) and eight humans (two control, three SS, and three NASH), using 80-MHz acoustic microscopy. The sum of percentage (SP) composition of FFAs (SP-FFAs) was also assessed.
Results
Median impedance of all FFAs was 0.7 (5 FFAs with impedance 0.7); 17 FFAs with impedance < 0.7 were classified as low-impedance group; and, 13 FFAs with impedance > 0.7 were classified as high-impedance group. The median impedance of the mouse liver decreased from control (1.715), to SS (1.68), to NASH (1.635) (control versus NASH, p = 0.039 without significant differences for the other comparisons, p ≥ 0.1). Similarly, the median impedance of human liver showed decreased from control (1.825), to SS (1.788), to NASH (1.76) (control versus SS, p = 0.023; control versus NASH, p = 0.003; SS versus NASH, p = 0.050). The ratio of SP-FFAs between the low-impedance and high-impedance groups showed an increase in both mice and humans, with significant differences in mice (control versus SS, p < 0.001; control versus NASH, p < 0.001; SS versus NASH, p = 0.003), without significant differences in humans (p ≥ 0.671).
Conclusion
Lower acoustic impedance based on the intrahepatic composition of FFAs may be characteristic of NASH.
中文翻译:
基于游离脂肪酸的低阻抗肝图像:非酒精性脂肪性肝炎(NASH)的特征性表现
摘要
背景
要检查小鼠和人类肝脏中非酒精性脂肪病的体外声学特性,以鉴定非酒精性脂肪性肝炎(NASH)。
方法
在十二种小鼠的35种游离脂肪酸(FFA,500 mmol / L)和经组织学诊断的肝脏样本中测量了声阻抗(×10 6 kg / m 2 / s)(四只对照组,四只简单脂肪变性[SS]和4个NASH)和8个人类(两个对照组,三个SS和三个NASH),使用80 MHz声学显微镜。还评估了FFA(SP-FFA)的百分比(SP)组成总和。
结果
所有FFA的中值阻抗为0.7(5个FFA的阻抗为0.7);17个阻抗<0.7的FFA被归为低阻抗组;将13个阻抗> 0.7的FFA归为高阻抗组。小鼠肝脏的平均阻抗从控制(1.715)降低,到SS(1.68),对NASH(1.635)(控制与NASH,p = 0.039,而不对其他比较显著差异,p ≥0.1)。同样,人肝的中位阻抗显示从对照组(1.825)降低到SS(1.788),再到NASH(1.76)(对照组与SSH,p = 0.023;对照组与NASH,p = 0.003; SS与NASH,p= 0.050)。低阻抗组和高阻抗组之间的SP-FFA比例在小鼠和人类中均表现出增加,但在小鼠中有显着差异(对照组与SS,p <0.001;对照组与NASH,p <0.001; SS与NASH ,p = 0.003),而不会在人体中(显著差异p)≥0.671。
结论
基于FFA肝内成分的较低声阻抗可能是NASH的特征。