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A SERS aptasensor for simultaneous multiple pathogens detection using gold decorated PDMS substrate.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.saa.2020.118103 Nuo Duan 1 , Mofei Shen 2 , Shuo Qi 2 , Wenyue Wang 3 , Shijia Wu 1 , Zhouping Wang 1
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.saa.2020.118103 Nuo Duan 1 , Mofei Shen 2 , Shuo Qi 2 , Wenyue Wang 3 , Shijia Wu 1 , Zhouping Wang 1
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An aptamer-based sensitive method was developed here for detection of multiple foodborne pathogens in food matrix by surface-enhanced Raman scattering (SERS) technology. Polydimethylsiloxane (PDMS) film was first prepared and then coated with gold nanoparticles (AuNP) to act as an active substrate for the enhancement of Raman scattering. The as-prepared Au-PDMS film was functionalized with specific pathogen aptamers (Apt) to capture the targets. In addition, aptamers functionalized AuNP integrated with Raman reporters (4-Mercaptobenzoic acid (4-MBA)/Nile blue A (NBA)) were fabricated as pathogen-specific SERS probes. In this scheme, pathogens were first captured by Apt-Au-PDMS film and then bind with SERS probes to allow the formation of a sandwich assay to complete the sensor module for the detection of multiple pathogens. With Vibrio parahaemolyticus and Salmonella typhimurium as model targets, this protocol can selectively detect 18 cfu/mL and 27 cfu/mL, respectively. Furthermore, this platform can be successfully applied to detect pathogens in seafood samples with recoveries ranging from 82.9% to 95.1%.
中文翻译：
SERS适体传感器，用于使用金色装饰的PDMS底物同时检测多种病原体。
本文开发了一种基于适体的灵敏方法，可通过表面增强拉曼散射（SERS）技术检测食物基质中多种食源性病原体。首先制备聚二甲基硅氧烷（PDMS）膜，然后用金纳米颗粒（AuNP）涂层，以用作增强拉曼散射的活性基质。将制备的Au-PDMS膜用特定的病原体适体（Apt）进行功能化，以捕获靶标。另外，将与拉曼报道分子（4-巯基苯甲酸（4-MBA）/尼罗蓝A（NBA））整合的适体功能化AuNP作为病原体特异性SERS探针。在该方案中，首先通过Apt-Au-PDMS膜捕获病原体，然后与SERS探针结合以形成夹心测定，从而完成用于检测多种病原体的传感器模块。以副溶血性弧菌和鼠伤寒沙门氏菌为模型靶标，该方案可以分别选择性检测18 cfu / mL和27 cfu / mL。此外，该平台可成功用于检测海鲜样品中的病原体，回收率在82.9％至95.1％之间。
更新日期：2020-01-23
中文翻译：
SERS适体传感器，用于使用金色装饰的PDMS底物同时检测多种病原体。
本文开发了一种基于适体的灵敏方法，可通过表面增强拉曼散射（SERS）技术检测食物基质中多种食源性病原体。首先制备聚二甲基硅氧烷（PDMS）膜，然后用金纳米颗粒（AuNP）涂层，以用作增强拉曼散射的活性基质。将制备的Au-PDMS膜用特定的病原体适体（Apt）进行功能化，以捕获靶标。另外，将与拉曼报道分子（4-巯基苯甲酸（4-MBA）/尼罗蓝A（NBA））整合的适体功能化AuNP作为病原体特异性SERS探针。在该方案中，首先通过Apt-Au-PDMS膜捕获病原体，然后与SERS探针结合以形成夹心测定，从而完成用于检测多种病原体的传感器模块。以副溶血性弧菌和鼠伤寒沙门氏菌为模型靶标，该方案可以分别选择性检测18 cfu / mL和27 cfu / mL。此外，该平台可成功用于检测海鲜样品中的病原体，回收率在82.9％至95.1％之间。
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