Examination of the transcriptomic activity of an organ at single cell resolution is one of the major breakthroughs in modern biology. Single cell RNA sequencing (scRNA-seq) is built on the paradigm-shifting work of Brady and Iscove [1], who described a method for generating microgrammes of cDNA from samples as small as a single cell almost 30 years ago. This discovery was followed closely by single cell transcriptomic analysis in neurons, accomplished by microinjecting primer, nucleotides and reverse transcriptase enzyme into dissociated cells [2]. Single cell sequencing has identified new mesenchymal lineage markers in early human development, allowing for the more precise identification and characterisation of mesenchymal subpopulations in lung development and disease http://bit.ly/2NpX6UY

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人类肺部发育的单细胞分析：了解间充质细胞是什么以及它们可能是什么


以单细胞分辨率检查器官的转录组活性是现代生物学的重大突破之一。单细胞 RNA 测序 (scRNA-seq) 建立在 Brady 和 Iscove [1] 的范式转变工作的基础上，他们在大约 30 年前描述了一种从小至单细胞的样本中生成微克 cDNA 的方法。这一发现紧随其后的是神经元的单细胞转录组分析，通过将引物、核苷酸和逆转录酶显微注射到解离的细胞中来完成[2]。单细胞测序在人类早期发育中发现了新的间充质谱系标记，从而可以更精确地识别和表征肺部发育和疾病中的间充质亚群 http://bit.ly/2NpX6UY