Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural production. In this study, an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin, trans-zeatin riboside, gibberellin A₃, indol-3-acetic acid, salicylic acid, abscisic acid, and jasmonic acid (JA) without any label. The separation was performed on an Agilent Explus Plus C₁₈ column by using methanol and water as mobile phases with gradient elution. The target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin, trans-zeatin riboside, indole-3-acetic acid, and via negative electrospray ionization for gibberellin₃, salicylic acid, abscisic acid, and JA. The limits of detection ranged from 0.0127 ng L⁻¹ for gibberellin A₃ (GA₃) to 33.26 ng L⁻¹ for JA and were lower than the currently reported values in literature. The proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and pods. The recoveries of the spiked phytohormones ranged from 80.20 to 102.56%. The contents of seven endogenous hormones varied specifically in different development stages of peanuts. This study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit, which are controlled by seven endogenous hormones.

开发一种高灵敏度，高可靠性的植物激素检测方法对研究植物激素和农业生产具有重要意义。在这项研究中，建立了一种超高效液相色谱-质谱/质谱法，用于分离和定量反玉米蛋白，反玉米蛋白核糖苷，赤霉素A ₃，吲哚-3-乙酸，水杨酸，脱落酸酸和茉莉酸（JA），没有任何标签。分离使用Agilent Explus Plus C ₁₈色谱柱，通过使用甲醇和水作为流动相进行梯度洗脱进行。通过正电喷雾电离对反式玉米蛋白，反式玉米蛋白进行质谱鉴定并确定目标化合物-玉米蛋白核糖苷，吲哚-3-乙酸，以及通过负电喷雾电离获得的赤霉素₃，水杨酸，脱落酸和JA。赤霉素A ₃（GA ₃）的检出限为0.0127 ng L ^-1到33.26 ng L ^-1JA的值低于目前文献报道的值。该方法用于花生陀螺和豆荚中植物激素的定性和定量分析。加标植物激素的回收率在80.20至102.56％之间。在花生的不同发育阶段，七种内源激素的含量特别不同。这项研究为激素提供了一种高度灵敏和选择性的检测方法，并阐明了由7种内源激素控制的绞股蓝和花生果实的生长和发育。