Three new pregnane glycosides in addition to four known compounds were isolated from the methylene chloride fraction of Caralluma hexagona Lavranos using bioassay-guided fractionation. The new compounds were identified as 12,20-di-O-benzoyl-3β,8β,12β,14β,20-pentahydroxy-(20R)-pregn-5-ene-3-O-β-d-glucopyranosyl-(1→4)-β-d-digitaloside (1), 3β,8β,14β,20-tetrahydroxy (20R)-pregn-5-ene-3-O-β-d-glucopyranosyl-(1→4)-O-β-d-digitaloside-20-O-β–d-glucopyranoside (2), 3β,8β,14β,20-tetrahydroxy-(20R)-pregn-5-ene-3-O-β-d-glucopyranosyl-(1→6)-O-β-d-glucopyranosyl-(1→4)-O-β-d-digitaloside-20-O-β–d-glucopyranoside (3), along with the known compounds luteolin 4`-O-neohesperidoside (4), apigenin-8-C-neohesperoside (5), β-sitosterol (6) and β-sitosterol glucoside (7). Preliminary studies of the crude methanolic extract and methylene chloride fraction showed inhibitory effects against α-glucosidase and pancreatic lipase. Among the isolated compounds, compound 5 showed the most potent α-glucosidase inhibition with IC₅₀ value of 0.82 ± 2.50 μM compared to acarbose (0.81 ± 0.86 μM). Whereas compound 1 showed the highest inhibitory activity on pancreatic lipase with an IC₅₀ value of 23.59 ± 2.49 μM compared to orlistat (7.41 ± 2.26 μM).

使用生物测定指导的分馏方法，从六倍体Carallumahexaa Lavranos的二氯甲烷馏分中分离出三种新的孕烷糖苷。新的化合物，分别为12,20二ø -苯甲酰基-3 β，8 β，12 β，14 β，20-pentahydroxy-（20 - [R ）-孕-5-烯-3- ø - β - d -glucopyranosyl-（1→4） - β - d -digitaloside （1）， 3 β，8 β，14 β，20四羟基（20 - [R ）-孕-5-烯-3- ö- β - d -glucopyranosyl-（1→4） - ø - β - d -digitaloside -20- ø - β - d吡喃葡萄糖苷（2），3 β，8个β，14 β，20-四羟基（20 - [R） -孕-5-烯-3- ø - β - d -glucopyranosyl-（1→6） - ø - β - d -glucopyranosyl-（1→4） - ø - β - d -digitaloside -20- ø - β - d-葡吡喃糖苷（3）以及已知化合物木犀草素4`- O-新芥子苷（4），芹菜素-8- C-新芥子苷（5），β-谷甾醇（6）和β-谷甾醇糖苷（7）。对粗甲醇提取物和二氯甲烷馏分的初步研究表明，其对α-葡萄糖苷酶和胰腺脂肪酶具有抑制作用。在分离的化合物中，与阿卡波糖（0.81±0.86μM）相比，化合物5表现出最有效的α-葡萄糖苷酶抑制作用，IC ₅₀值为0.82±2.50μM。而复合与奥利司他（7.41±2.26μM）相比，图1显示了对胰腺脂肪酶的最高抑制活性，IC ₅₀值为23.59±2.49μM。