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Multivariate quantitative analysis of quality trend based on non-volatile characteristic components in different Panax notoginseng samples using HPLC.
Journal of Pharmaceutical and Biomedical Analysis ( IF 3.1 ) Pub Date : 2020-01-23 , DOI: 10.1016/j.jpba.2020.113127
Chao Li 1 , Yunhua Qin 1 , Qianxu Yang 1 , Junheng You 1 , Zhihua Liu 1 , Jingmei Han 1 , E'xian Li 2 , Chengming Zhang 1
Affiliation  

In order to identify real and fake Panax notoginseng samples, the high performance liquid chromatography (HPLC) was used to analyze P. notoginseng samples of non-volatile characteristic components in P. notoginseng powder samples with 10 %, 30 %, 50 % ratio, combined with principal component analysis - mahalanobis distance (PCA-MD). The results showed that: (1) The PCA analysis showed that mahalanobis with different stem and leaf powder were divided into four categories, and the gravity center of the pattern (classification) showed that the long axis of the trend chart gradually increased with the increase of the proportion of the stem and leaf powder, and the trend chart was gradually away from the control group; (2) The mahalanobis distance indicated that the range of mahalanobis distance fluctuation (upper limit) of several P. notoginseng powder samples is 395.03∼48252.70. The larger the percentage of the stem and leaf powder in P. notoginseng samples, it is farther away from the normal P. notoginseng samples. It indicated that the product of P. notoginseng samples can be evaluated with the mahalanobis distance. The results of this study can be applied to identification and homogenization evaluation of P. notoginseng between real and fake samples.

中文翻译:

基于三七不同样品中的非挥发性特征成分的HPLC多质量趋势定量分析。

为了鉴别真假三七样品,使用高效液相色谱(HPLC)分析三七粉末样品中非挥发性特征成分的三七样品,其比例为10%,30%,50%,结合主成分分析-马氏距离(PCA-MD)。结果表明:(1)PCA分析表明,不同茎和叶粉的马哈拉诺比斯分为四类,且图案的重心(分类)表明趋势图的长轴随着增加而逐渐增加。茎叶粉的比例,趋势图逐渐远离对照组。(2)马氏距离表示马氏距离波动的范围(上限)为P。三七粉末样品为395.03〜48252.70。三七样品中茎和叶粉的百分比越大,离正常三七样品越远。这表明可以用马哈拉诺比斯距离评估三七的样品产物。本研究结果可用于真假样品中三七的鉴定和均质化评价。
更新日期:2020-01-23
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