Aryl Hydrocarbon Receptor as a target for lycopene preventing DEHP-induced spermatogenic disorders.,Journal of Agricultural and Food Chemistry

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Aryl Hydrocarbon Receptor as a target for lycopene preventing DEHP-induced spermatogenic disorders.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-04-02 , DOI: 10.1021/acs.jafc.9b07795
Yi Zhao ₁ , Jia Lin _{1,

2} , Milton Talukder _{1,

3} , Shi-Yong Zhu ₁ , Mu-Zi Li ₁ , Hao-Ran Wang ₁ , Jin-Long Li _{1,

4,

5}

Affiliation

Di (2-ethylhexyl) phthalate (DEHP) is widely used as a plasticizer to improve its flexibility and workability. Lycopene (LYC) is a natural compound and has promising preventive potentials, especially anti-reproductive toxicity, but specific underlying mechanism has yet to be fully defined. Our study investigated the effects of LYC on DEHP-induced spermatogenesis disturbance. The male ICR mice were treated with DEHP (500 mg/kg BW/day or 1000 mg/kg BW/day) and/or LYC (5 mg/kg BW/day) for 28 days. Our results indicated that LYC could relieve DEHP-induced injury of seminiferous tubules and spermatogenic cells, swelling of endoplasmic reticulum (ER) and the increase of mitochondria. LYC prevented an increasing of the levels of nuclear damage to DNA and deformity rate, and a decreasing of the values of sperm motility, number and density. Moreover, LYC treatment decreased DEHP-induced nuclear accumulation of AHR and ARNT, and the expression of their downstream target genes (CYP1A1, CYP1A2 and CYP1B1) were markedly reduced to normal in the LYC treatment group. Our study showed that LYC could prevent the potential against DEHP-induced spermatogenic disorders via AHR/ARNT signaling system. This study provided the new evidence of AHR as a target for LYC which could prevent the DEHP-induced toxicity.

中文翻译：

芳烃受体作为番茄红素的靶标，可预防DEHP引起的生精障碍。

邻苯二甲酸二（2-乙基己基）酯（DEHP）被广泛用作增塑剂，以提高其柔韧性和可加工性。番茄红素（LYC）是一种天然化合物，具有令人鼓舞的预防潜力，尤其是抗生殖毒性，但具体的潜在机制尚未完全阐明。我们的研究调查了LYC对DEHP诱导的生精障碍的影响。用DEHP（500mg / kg BW /天或1000mg / kg BW /天）和/或LYC（5mg / kg BW /天）处理雄性ICR小鼠28天。我们的结果表明，LYC可以缓解DEHP引起的生精小管和生精细胞损伤，内质网肿胀和线粒体增加。LYC阻止了对DNA的核损伤水平的提高和畸形率的降低，以及精子活力，数量和密度的降低。此外，LYC治疗组降低了DEHP诱导的AHR和ARNT的核积累，并且下游靶基因（CYP1A1，CYP1A2和CYP1B1）的表达在LYC治疗组中显着降低。我们的研究表明，LYC可以通过AHR / ARNT信号传导系统预防DEHP诱导的生精障碍。这项研究提供了AHR作为LYC靶标的新证据，可以预防DEHP诱导的毒性。

更新日期：2020-04-03

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