Aerobic performance in fish is linked to individual and population fitness and can be impacted by anthropogenic contaminants. Exposure to some engineered nanomaterials, including silver nanoparticles (nAg), reduces rates of oxygen consumption in some fish species, but the underlying mechanisms remain unclear. In addition, their effects on swim performance have not been studied. Our aim was to quantify the impact of exposure to functionalized nAg on aerobic scope and swim performance in rainbow trout (Oncorhychus mykiss) and to characterize the contribution of changing rates of protein synthesis to these physiological endpoints. Fish were exposed for 48 h to 5 nm polyvinylpyrolidone-functionalized nAg (nAgPVP; 100 μg L⁻¹) or 0.22 μg L⁻¹ Ag⁺ (as AgNO₃), which was the measured quantity of Ag released from the nAgPVP over that time period. Aerobic scope, critical swimming speed (U_crit), and fractional rates of protein synthesis (K_s), were then assessed, along with indicators of osmoregulation and cardiotoxicity. Neither nAgPVP, nor Ag⁺ exposure significantly altered aerobic scope, its component parts, or swim performance. K_s was similarly unaffected in 8 tissue types, though it tended to be lower in liver of nAgPVP treated fish. The treatments tended to decrease gill Na⁺/K⁺-ATPase activity, but effects were not significant. The latter results suggest that a longer or more concentrated nAgPVP exposure may induce significant effects. Although this same formulation of nAgPVP is bioactive in other fish, it had no effects on rainbow trout under the conditions tested. Such findings on common model animals like trout may thus misrepresent the safety of nAg to more sensitive species.

鱼的有氧性能与个体和种群的适应度有关，并可能受到人为污染物的影响。暴露于某些工程纳米材料中，包括银纳米颗粒（nAg），可以降低某些鱼类的耗氧率，但其潜在机制尚不清楚。另外，尚未研究它们对游泳成绩的影响。我们的目的是量化功能化nAg暴露对虹鳟（Oncorhychus mykiss）有氧运动范围和游泳性能的影响，并表征蛋白质合成速率变化对这些生理终点的影响。将鱼暴露于5 nm的聚乙烯吡咯烷酮官能化的nAg（nAgPVP; 100μgL ^-1）或0.22μgL ^-1 Ag ^+中48小时（如AgNO ₃），这是在该时间段内从nAgPVP释放的测得的Ag量。然后评估有氧运动范围，临界游泳速度（U _crit）和蛋白质合成分数（K _s），以及渗透压和心脏毒性指标。nAgPVP或Ag ⁺暴露均不会显着改变有氧范围，其组成部分或游泳性能。在8种组织类型中，K _s同样不受影响，尽管在用nAgPVP处理的鱼的肝脏中K _s倾向于较低。治疗倾向于减少decrease Na ⁺ / K ⁺-ATP酶活性，但作用不明显。后者的结果表明，更长或更长时间的nAgPVP暴露可能引起显着影响。尽管相同的nAgPVP配方在其他鱼类中具有生物活性，但在测试条件下对虹鳟鱼没有影响。因此，在常见模型动物（如鳟鱼）上的此类发现可能会歪曲nAg对更敏感物种的安全性。