The current study was designed to investigate the physiochemical properties, protein and metabolite profiles of muscle exudate obtained from chicken breast fillets affected by wooden breast (WB) myopathy. Twenty-four fillets were categorized into varying degrees of WB condition including normal, moderate and severe. Results indicated that exudate loss, free hemoglobin concentration, protein and lipid oxidation were affected by WB myopathy. Electrophoresis analysis showed eight distinct protein bands of differential relative abundance in WB samples compared with the normal, and the identified proteins were mostly involved in carbohydrate metabolic process. 1H nuclear magnetic resonance-based metabolomics identified eleven metabolites including amino acids, nucleotides and organic acid as the most influential metabolites affected by WB myopathy. Overall, this study shows differential molecular profiles of myopathic chicken muscle exudate, and provides a valuable resource for further recognition of WB myopathy.

中文翻译：

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受木制乳腺肌病影响的鸡肉肌肉渗出液的理化特性，蛋白质和代谢产物概况。

当前的研究旨在研究从受木胸肌病影响的鸡胸肉中获得的肌肉渗出液的理化特性，蛋白质和代谢产物概况。24个鱼片被分类为不同程度的WB状况，包括正常，中度和严重。结果表明，WB肌病影响渗出液损失，游离血红蛋白浓度，蛋白质和脂质氧化。电泳分析显示，与正常样品相比，WB样品中有八个不同的相对丰度不同的蛋白带，并且鉴定出的蛋白主要参与碳水化合物的代谢过程。基于1H核磁共振的代谢组学确定了11种代谢物，包括氨基酸，核苷酸和有机酸，是受WB肌病影响最大的代谢物。