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Screening, identification and application of soil bacteria with nematicidal activity against root-knot nematode (Meloidogyne incognita) on tomato.
Pest Management Science ( IF 3.8 ) Pub Date : 2020-02-20 , DOI: 10.1002/ps.5759
Guangying Liu 1, 2 , Xu Lin 1, 2 , Shuangyu Xu 1, 2 , Guang Liu 1, 2 , Feng Liu 1, 2 , Wei Mu 1, 2
Affiliation  

BACKGROUND Root-knot nematodes (Meloidogyne spp.) are soilborne pathogens that can cause great damage to and economic loss of crops globally. Owing to the high toxicity of chemicals toward humans and the environment, the use of biocontrol bacteria, a promising method for controlling root-knot nematodes, has gained attention. RESULTS To screen novel bacterial strains for their ability to control root-knot nematodes, 106 bacterial strains were isolated from soil. Eight of the obtained isolates exhibited satisfactory nematicidal activity against Meloidogyne incognita at 2-fold dilutions (approximately 3 × 1012  CFU mL-1 ) after 12 h of exposure. Based on their physiological, biochemical and molecular (16S rRNA and gyrB gene sequences) characteristics, the eight strains were identified as Bacillus halotolerans, B. kochii, B. oceanisediminis, B. pumilus, B. toyonensis, B. cereus, Pseudomonas aeruginosa and B. pseudomycoides. In greenhouse and field experiments, the eight isolates suppressed M. incognita up to 69.96% compared to the control. Additionally, the yield of tomato increased 1.4-26.1% over that of the control. CONCLUSION The strains of B. halotolerans DDWA, B. kochii DDWB, B. oceanisediminis DDWC and B. pseudomycoides JNC have potential to control M. incognita, which has not been previously reported. © 2020 Society of Chemical Industry.

中文翻译:

对番茄根结线虫(Meloidogyne incognita)具有杀线虫活性的土壤细菌的筛选,鉴定和应用。

背景技术根结线虫(Meloidogyne spp。)是土壤传播的病原体,可对全球范围内的农作物造成巨大损害并造成经济损失。由于化学药品对人类和环境的高毒性,使用生物防治细菌作为一种控制根结线虫的有前途的方法,已引起人们的关注。结果为了筛选新型细菌菌株控制根结线虫的能力,从土壤中分离出106株细菌。暴露的12小时后,在2倍稀释度(约3×1012 CFU mL-1)下,获得的八株分离株对南方根结线虫表现出令人满意的杀线虫活性。根据它们的生理,生化和分子特征(16S rRNA和gyrB基因序列),确定了这8个菌株,分别为卤化芽孢杆菌,科氏芽孢杆菌,大肠弯曲杆菌,海洋芽孢杆菌。pumilus,B。toyonensis,蜡状芽孢杆菌,铜绿假单胞菌和假双歧杆菌 在温室和田间实验中,与对照相比,这八种分离物抑制了隐孢子虫,最高可抑制69.96%。此外,番茄的产量比对照增加了1.4-26.1%。结论B. halotolerans DDWA,B。kochii DDWB,B。oceaniseiminisnis DDWC和B. pseudomycoides JNC的菌株具有控制隐孢分枝杆菌的潜力,以前尚未见报道。©2020年化学工业协会。oceaniseiminis DDWC和假双歧杆菌JNC具有控制隐孢分枝杆菌的潜力,以前尚未见报道。©2020年化学工业协会。oceaniseiminis DDWC和假双歧杆菌JNC具有控制隐孢分枝杆菌的潜力,以前尚未见报道。©2020年化学工业协会。
更新日期:2020-02-20
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