BACKGROUND Arthrofibrosis is a painful and restraining complication that occurs after about 10% of total knee arthroplasty and cruciate ligament surgery. The pathogenesis of arthrofibrosis has not yet been fully understood. Stress signals stimulate immune cells, and fibroblast differentiates into myofibroblast, which produce a large amount of collagen. Xylosyltransferases also appear to be involved in these pathways. They catalyze proteoglycan biosynthesis, which is involved in tissue remodeling and myofibroblast differentiation. The aim of this study was to investigate the relationship between the disease arthrofibrosis and the expression of the two isoforms of xylosyltransferases I and II. METHODS Tissue samples from 14 patients with arthrofibrosis were compared with tissue samples from seven healthy controls. The xylosyltransferases were detected by immunohistochemistry. The tissues were divided into four different areas of interest: vessels, synovialis, cell-poor and cell-rich fibrosis, or cell-poor and cell-rich areas in the control group. A quantification of the results was performed by modification of the immunoreactive score according to Remmele and Stegner. RESULTS Xylosyltransferase I was expressed in the various tissue types at varying rates. Xylosyltransferase I expression was considerably and significantly stronger than that of xylosyltransferase II. The following sequences of xylosyltransferase I and xylosyltransferase II expression were determined as follows: vessels >> cell-rich fibrosis > cell-poor fibrosis > synovialis. A positive correlation between the number of positive fibroblasts and the immunoreactive scoring system (IRS) was documented. CONCLUSIONS The significant positive correlation of xylosyltransferase -I expression with increasing number of fibroblasts demonstrates a high myofibroblast differentiation rate, which implies a gradual event as the pathogenesis of arthrofibrosis.

中文翻译：

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木糖基转移酶 I 和 II 的表达及其在关节纤维化发病机制中的作用。


背景技术关节纤维化是一种痛苦且限制性的并发症，大约10%的全膝关节置换术和十字韧带手术后发生。关节纤维化的发病机制尚未完全清楚。压力信号刺激免疫细胞，成纤维细胞分化为肌成纤维细胞，产生大量胶原蛋白。木糖基转移酶似乎也参与这些途径。它们催化蛋白多糖生物合成，参与组织重塑和肌成纤维细胞分化。本研究的目的是探讨关节纤维化疾病与木糖基转移酶 I 和 II 两种亚型表达之间的关系。方法 将 14 名关节纤维化患者的组织样本与 7 名健康对照者的组织样本进行比较。通过免疫组织化学检测木糖基转移酶。将组织分为四个不同的感兴趣区域：血管、滑膜、缺乏细胞和富含细胞的纤维化，或对照组的缺乏细胞和富含细胞的区域。根据 Remmele 和 Stegner 的规定，通过修改免疫反应评分来对结果进行量化。结果 木糖基转移酶 I 在各种组织类型中以不同的速率表达。木糖基转移酶I的表达明显强于木糖基转移酶II的表达。木糖基转移酶I和木糖基转移酶II表达的以下顺序确定如下：血管>>富含细胞的纤维化>细胞贫乏的纤维化>滑膜。阳性成纤维细胞数量与免疫反应评分系统（IRS）之间存在正相关关系。 结论 木糖基转移酶-I表达与成纤维细胞数量增加呈显着正相关，表明肌成纤维细胞分化率较高，这意味着关节纤维化的发病机制是渐进的。