BACKGROUND The total bacterial community plays an important role in aquatic ecosystems. In this study, bacterial communities and diversity along the shores of the Upper Gulf of Thailand were first characterized. The association between bacterial communities and types of land use was also evaluated. RESULTS The bacterial communities and diversity of seawater in the Upper Gulf of Thailand, with regard to types of land use, were first revealed by using Illumina next-generation sequencing. A total of 4953 OTUs were observed from all samples in which 554 OTUs were common. The bacterial communities in sampling sites were significantly different from each other. The run-off water from three types of land use significantly affected the community richness and diversity of marine bacteria. Aquaculture sites contained the highest levels of community richness and diversity, followed by mangrove forests and tourist sites. Seawater physicochemical parameters including salinity, turbidity, TSS, total N, and BOD5, were significantly different when grouped by land use. The bacterial communities were mainly determined by salinity, total N, and total P. The species richness estimators and OTUs were positively correlated with turbidity. The top ten most abundant phyla and genera as well as the distribution of bacterial classes were characterized. The Proteobacteria constituted the largest proportions in all sampling sites, ranging between 67.31 and 78.80%. The numbers of the Marinobacterium, Neptuniibacter, Synechococcus, Candidatus Thiobios, hgcI clade (Actinobacteria), and Candidatus Pelagibacter were significantly different when grouped by land use. CONCLUSIONS Type of land use significantly affected bacterial communities and diversity along the Upper Gulf of Thailand. Turbidity was the most influential parameter affecting the variation in bacterial community composition. Salinity, total N, and P were the ones of the important factors that shaped the bacterial communities. In addition, the variations of bacterial communities from site-to-site were greater than within-site. The Proteobacteria, Bacteroidetes, Actinobacteria, Cyanobacteria, Verrucomicrobia, Euryarchaeota, Planctomycetes, Firmicutes, Deep Sea DHVEG-6, and Marinimicrobia were the most and common phyla distributed across the Upper Gulf of Thailand.

中文翻译：

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Illumina下一代测序平台对泰国上海湾的海洋细菌群落进行了评估。

背景技术总细菌群落在水生生态系统中起重要作用。在这项研究中，首先描述了泰国上海湾沿岸细菌群落和多样性。还评估了细菌群落与土地利用类型之间的关联。结果首先通过Illumina新一代测序技术揭示了泰国上海湾的细菌群落和海水多样性，涉及土地利用类型。从所有样品中总共观察到4953个OTU，其中554个OTU是常见的。采样地点的细菌群落彼此之间存在显着差异。来自三种土地利用类型的径流水显着影响了海洋细菌的群落丰富性和多样性。水产养殖场所的社区丰富度和多样性最高，其次是红树林和旅游胜地。按土地用途分组时，包括盐度，浊度，TSS，总氮和BOD5在内的海水理化参数显着不同。细菌群落主要由盐度，总氮和总磷决定。物种丰富度估算值和OTU与浊度呈正相关。确定了前十名最丰富的门和属以及细菌种类的分布。在所有采样点中，变形杆菌占最大比例，介于67.31％和78.80％之间。marinobacterium，Neptuniibacter，Synechococcus，Thiobios念珠菌，hgcI进化枝（放线菌）的数量，按土地利用分组时，百日咳杆菌和念珠菌之间存在显着差异。结论土地利用类型严重影响了泰国上海湾沿岸的细菌群落和多样性。浊度是影响细菌群落组成变化的最有影响的参数。盐度，总氮和磷是影响细菌群落的重要因素之一。此外，不同地点之间细菌群落的变化大于内部地点。变形杆菌，拟杆菌，放线菌，蓝细菌，Verrucomicrobia，Euryarchaeota，Planctomycetes，Firmicutes，Deep Sea DHVEG-6和Marinimicrobia是分布在泰国上海湾的最多，最常见的门。结论土地使用类型严重影响了泰国上海湾沿岸的细菌群落和多样性。浊度是影响细菌群落组成变化的最有影响的参数。盐度，总氮和磷是影响细菌群落的重要因素之一。此外，不同地点之间细菌群落的变化大于内部地点。变形杆菌，拟杆菌，放线菌，蓝细菌，Verrucomicrobia，Euryarchaeota，Planctomycetes，Firmicutes，Deep Sea DHVEG-6和Marinimicrobia是分布在泰国上海湾的最多，最常见的门。结论土地使用类型严重影响了泰国上海湾沿岸的细菌群落和多样性。浊度是影响细菌群落组成变化的最有影响的参数。盐度，总氮和磷是影响细菌群落的重要因素之一。此外，不同地点之间细菌群落的变化大于内部地点。变形杆菌，拟杆菌，放线菌，蓝细菌，Verrucomicrobia，Euryarchaeota，Planctomycetes，Firmicutes，Deep Sea DHVEG-6和Marinimicrobia是分布在泰国上海湾的最多，最常见的门。P和P是影响细菌群落的重要因素之一。此外，不同地点之间细菌群落的变化大于内部地点。变形杆菌，拟杆菌，放线菌，蓝细菌，Verrucomicrobia，Euryarchaeota，Planctomycetes，Firmicutes，Deep Sea DHVEG-6和Marinimicrobia是分布在泰国上海湾的最多，最常见的门。P和P是影响细菌群落的重要因素。此外，不同地点之间细菌群落的变化大于内部地点。变形杆菌，拟杆菌，放线菌，蓝细菌，Verrucomicrobia，Euryarchaeota，Planctomycetes，Firmicutes，Deep Sea DHVEG-6和Marinimicrobia是分布在泰国上海湾的最多，最常见的门。