A human stem cell–based microrobot system for knee cartilage regeneration was designed with consideration of clinical trials. Targeted cell delivery by a magnetically actuated microrobot with a porous structure is a promising technique to enhance the low targeting efficiency of mesenchymal stem cell (MSC) in tissue regeneration. However, the relevant research performed to date is only in its proof-of-concept stage. To use the microrobot in a clinical stage, biocompatibility and biodegradation materials should be considered in the microrobot, and its efficacy needs to be verified using an in vivo model. In this study, we propose a human adipose–derived MSC–based medical microrobot system for knee cartilage regeneration and present an in vivo trial to verify the efficacy of the microrobot using the cartilage defect model. The microrobot system consists of a microrobot body capable of supporting MSCs, an electromagnetic actuation system for three-dimensional targeting of the microrobot, and a magnet for fixation of the microrobot to the damaged cartilage. Each component was designed and fabricated considering the accessibility of the patient and medical staff, as well as clinical safety. The efficacy of the microrobot system was then assessed in the cartilage defect model of rabbit knee with the aim to obtain clinical trial approval.

中文翻译：

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基于人体脂肪的间充质干细胞医学微型机器人系统，用于体内膝关节软骨再生

考虑临床试验设计了基于人类干细胞的微型机器人系统，用于膝关节软骨再生。具有多孔结构的磁致微型机器人对细胞的靶向递送是增强组织再生中间充质干细胞（MSC）的低靶向效率的有前途的技术。但是，迄今为止进行的相关研究仅处于概念验证阶段。为了在临床阶段使用微型机器人，应该在微型机器人中考虑生物相容性和生物降解材料，并且需要使用体内模型来验证其有效性。在这项研究中，我们提出了一种基于人类脂肪的基于MSC的医用微型机器人系统，用于膝关节软骨的再生，并提出了一项体内试验，以验证使用软骨缺损模型的微型机器人的功效。微型机器人系统由能够支持MSC的微型机器人主体，用于微型机器人三维定向的电磁致动系统以及用于将微型机器人固定到受损软骨的磁体组成。考虑到患者和医护人员的可及性以及临床安全性，设计和制造每个组件。然后在兔膝关节软骨缺损模型中评估了微型机器人系统的功效，旨在获得临床试验批准。以及临床安全性。然后在兔膝关节软骨缺损模型中评估了微型机器人系统的功效，旨在获得临床试验批准。以及临床安全性。然后在兔膝关节软骨缺损模型中评估了微型机器人系统的功效，旨在获得临床试验批准。