Late embryogenesis abundant (LEA) proteins comprise a large family that play important roles in the regulation of abiotic stresses. No in-depth analysis of LEA genes has been performed in grapevine. In this study, we analyzed a total of 52 putative LEA genes in grapevine at genomic and transcriptomic levels, compiled expression profiles of four selected VamLEA genes under cold and osmotic stress and studied potential function of VamDHN3 gene in grapevine callus. The 52 LEA proteins can be classified into seven phylogenetic groups. RNA-seq and qRT-PCR results demonstrated that a total of 16 and 23 VamLEA genes were upregulated under cold and osmotic stress. Overexpression of VamDHN3 enhances the stability of cell membrane in grapevine callus, suggesting VamDHN3 is involved in osmotic regulation. These results formed fundamental knowledge for further analysis of biological roles of LEA genes in grapevine's adaption to abiotic stresses.

中文翻译：

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葡萄LEA基因家族响应渗透胁迫和冷胁迫的转录组学分析，以及VamDHN3基因的功能分析。

晚期胚胎发生丰富 (LEA) 蛋白包含一个大家族，在非生物胁迫的调节中发挥重要作用。尚未对葡萄树中的 LEA 基因进行深入分析。在这项研究中，我们在基因组和转录组水平上分析了葡萄树中总共 52 个假定的 LEA 基因，编译了四个选定的 VamLEA 基因在冷和渗透胁迫下的表达谱，并研究了 VamDHN3 基因在葡萄愈伤组织中的潜在功能。52 种 LEA 蛋白可分为七个系统发育组。RNA-seq 和 qRT-PCR 结果表明，在冷胁迫和渗透胁迫下，共有 16 和 23 个 VamLEA 基因被上调。VamDHN3 的过表达增强了葡萄愈伤组织细胞膜的稳定性，表明 VamDHN3 参与渗透压调节。