Abstract In recent years, production of biosynthesized human milk oligosaccharides (HMOs) has become scalable to industrial standards. As a result, infant formula fortified with 2’‐fucosyllactose (2’‐FL), the most abundant HMO in human breast milk, is now commercially available. 2’‐FL and 3‐fucosyllactose (3‐FL), another abundant HMO, are thought to be beneficial for infant health and development. Products containing HMOs are projected to expand in the future, showing the need for robust, easily applicable analytical methods for the quantitative assessment of HMOs in different food applications. We present here a validated high‐performance liquid chromatography method for the quantification of 2’‐FL and 3‐FL in whole milk, infant formula, and cereal bars. The sample preparation was simple dispersion and extraction of the sample. The samples were analyzed by hydrophilic interaction liquid chromatography with refractive index detection and a runtime of 19 min. The method had a high degree of linearity (R 2 > 0.9995) in the range 0.2 to 12 mg/mL. The recovery for 2’‐FL was 88% to 105% and for 3‐FL 94% to 112%. The limit of detection (LOD) for whole milk was 0.1 mg/mL for 2’‐FL and 0.2 mg/mL for 3‐FL. In infant formula and cereal bars, the LOD was 0.6 mg/g for both 2’‐FL and 3‐FL. To show the practical application of this method, it was successfully utilized in stability studies of 2’‐FL and 3‐FL in whole milk, UHT milk, and yoghurt. The method provides a means of simultaneous and robust quantification of 2’‐FL and 3‐FL in various food matrices with high accuracy and high reproducibility. Practical Application 2’‐Fucosyllactose (2’‐FL) and 3‐fucosyllactose (3‐FL) are two of the most abundant human milk oligosaccharides (HMOs) present in human breast milk. We present a fast HPLC method for the robust quantification of these two compounds in infant formula, whole milk, UHT milk, cereal bars, and yoghurt. This method can easily be set up by food producers and researchers to analyze the dosage of 2’‐FL and 3‐FL in their product or perform shelf life studies in different food applications.

中文翻译：

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通过具有折光率检测的高效液相色谱法定量不同食品应用中的人乳寡糖 2'-岩藻糖基乳糖和 3-岩藻糖基乳糖

摘要 近年来，生物合成人乳寡糖 (HMO) 的生产已达到工业标准。因此，添加 2'-岩藻糖基乳糖 (2'-FL) 的婴儿配方奶粉是母乳中含量最丰富的 HMO，现已上市。2'-FL 和 3-岩藻糖基乳糖 (3-FL) 是另一种丰富的 HMO，被认为有益于婴儿的健康和发育。预计未来含有 HMO 的产品将会扩大，这表明需要稳健、易于应用的分析方法来对不同食品应用中的 HMO 进行定量评估。我们在此介绍了一种经过验证的高效液相色谱方法，用于定量全脂牛奶、婴儿配方奶粉和谷物棒中的 2'-FL 和 3-FL。样品制备是简单的样品分散和萃取。样品通过亲水相互作用液相色谱进行分析，该色谱具有折射率检测器，运行时间为 19 分钟。该方法在 0.2 至 12 mg/mL 范围内具有高度线性 (R 2 > 0.9995)。2'-FL 的回收率为 88% 至 105%，3-FL 的回收率为 94% 至 112%。全脂牛奶的检测限 (LOD) 2'-FL 为 0.1 mg/mL，3-FL 为 0.2 mg/mL。在婴儿配方奶粉和谷物棒中，2'-FL 和 3-FL 的 LOD 均为 0.6 mg/g。为了展示该方法的实际应用，它已成功用于全脂牛奶、UHT 牛奶和酸奶中 2'-FL 和 3-FL 的稳定性研究。该方法提供了一种对各种食品基质中 2'-FL 和 3-FL 进行同步和稳健定量的方法，具有高精度和高重现性。实际应用 2'-岩藻糖基乳糖 (2'-FL) 和 3-岩藻糖基乳糖 (3-FL) 是母乳中含量最丰富的两种母乳低聚糖 (HMO)。我们提出了一种快速 HPLC 方法，用于对婴儿配方奶粉、全脂牛奶、UHT 牛奶、谷物棒和酸奶中的这两种化合物进行稳健定量。食品生产商和研究人员可以轻松建立此方法，以分析其产品中 2'-FL 和 3-FL 的剂量，或在不同食品应用中进行保质期研究。