Bacterial species in the plant-beneficial-environmental branch of Alcaligenes characterize an important part of rhizosphere microbes. The effects of plant growth-promoting Alcaligenes sp. AZ9 (accession #KU494828) on soil status and maize (Zea mays L.) performance were evaluated in single or combined applications with soil amendments, such as biochar (BC) prepared through pyrolysis of maize straw at 300 ºC, rock phosphate enriched compost (CM), and humic acid (HA) in two wirehouse experiments under natural environment. Qualitative P solubilization of Alcaligenes sp. AZ9 revealed the appearance of a halo zone up to 19.1 mm in agar medium with P solubilizing index 4.27 and P solubilizing efficiency 327%. Alcaligenes sp. AZ9 showed solubilization of P up to 30.85 µg mL−1 by lowering pH of medium from 7.0 to 5.25. The application of Alcaligenes sp. AZ9 significantly improved soil biological attributes, such as bacterial colony forming unit by 20.5%, soil organic carbon by 10.3%, soil organic matter by 5.1%, and water content of saturated soil by 5.7%. The combined application of Alcaligenes sp. AZ9 with either BC, CM, or HA or with their mixture (BC plus CM plus HA) significantly increased the above biological attributes of soil than the application of Alcaligenes sp. AZ9 alone. Moreover, the integrated application of Alcaligenes sp. AZ9 with BC, CM, and HA significantly improved growth and yield attributes of maize in terms of root length (26%), plant height (39%), shoot fresh biomass (31%), shoot dry biomass (32%), root fresh biomass (30%), root dry biomass (34%), 100-grains weight (26%), stover yield (28%), and grain yield (19%). Similarly, the integrated application of Alcaligenes sp. AZ9 with BC, CM, and HA promoted N, P, and K in maize grain concentration by 12%, 11%, and 11%, respectively, compared with the application of organic amendments without Alcaligenes sp. AZ9. Combined application of Alcaligenes sp. AZ9 with BC, CM, and HA could be an effective strategy to improve plant growth, nutrients uptake, and yield of maize in the context of sustainable agriculture. In future research, the effects of integrated application of Alcaligenes sp. AZ9 with BC, CM, and/or HA should be further evaluated under field conditions.

中文翻译：

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有机修正物与产碱杆菌的综合应用。AZ9 提高了玉米（Zea mays）的营养吸收和产量

产碱菌的植物有益环境分支中的细菌物种是根际微生物的重要组成部分。植物促生长产碱杆菌的作用。AZ9（登录号#KU494828）对土壤状况和玉米（Zea mays L.）性能的评估在单独或与土壤改良剂的组合应用中进行评估，例如通过在 300 ºC 下通过玉米秸秆热解制备的生物炭（BC）、富含磷酸盐的堆肥（ CM) 和腐殖酸 (HA) 在自然环境下的两个线材实验中。产碱杆菌的定性 P 溶解。AZ9 显示在琼脂培养基中出现长达 19.1 毫米的晕圈，磷溶解指数为 4.27，磷溶解效率为 327%。产碱菌属 通过将培养基的 pH 值从 7.0 降低到 5.25，AZ9 可将 P 溶解到 30.85 µg mL-1。产碱杆菌的应用。AZ9显着改善土壤生物属性，如细菌菌落形成单位提高20.5%，土壤有机碳提高10.3%，土壤有机质提高5.1%，饱和土壤含水量提高5.7%。产碱杆菌的联合应用。AZ9 与 BC、CM 或 HA 或它们的混合物（BC 加 CM 加 HA）显着增加了土壤的上述生物属性，而不是 Alcaligenes sp。AZ9一个人。此外，产碱杆菌的综合应用。含 BC、CM 和 HA 的 AZ9 在根长 (26%)、株高 (39%)、地上部鲜生物量 (31%)、地上部干生物量 (32%)、根系方面显着改善了玉米的生长和产量属性新鲜生物量 (30%)、根干生物量 (34%)、百粒重 (26%)、秸秆产量 (28%) 和谷物产量 (19%)。同样，产碱杆菌的综合应用。与施用不含产碱杆菌属的有机改良剂相比，含 BC、CM 和 HA 的 AZ9 分别将玉米籽粒中的 N、P 和 K 浓度提高了 12%、11% 和 11%。AZ9。产碱杆菌的联合应用。具有 BC、CM 和 HA 的 AZ9 可能是在可持续农业背景下改善玉米植物生长、养分吸收和产量的有效策略。在未来的研究中，产碱杆菌综合应用的效果。应在现场条件下进一步评估具有 BC、CM 和/或 HA 的 AZ9。在可持续农业的背景下，玉米的养分吸收和产量。在未来的研究中，产碱杆菌综合应用的效果。应在现场条件下进一步评估具有 BC、CM 和/或 HA 的 AZ9。在可持续农业的背景下，玉米的养分吸收和产量。在未来的研究中，产碱杆菌综合应用的效果。应在现场条件下进一步评估具有 BC、CM 和/或 HA 的 AZ9。