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A High Sensitive, Reproducible and Disposable Immunosensor for Analysis of SOX2
Electroanalysis ( IF 2.7 ) Pub Date : 2020-01-22 , DOI: 10.1002/elan.201900557 Nur Tarimeri 1 , Mustafa Kemal Sezgintürk 2
Electroanalysis ( IF 2.7 ) Pub Date : 2020-01-22 , DOI: 10.1002/elan.201900557 Nur Tarimeri 1 , Mustafa Kemal Sezgintürk 2
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In this study, an ITO (indium tin oxide) based biosensor was constructed to detect SOX2. SOX2 helps the regulation of cell pluripotency and is closely related to early embryonic development, neural and sexual differentiation. SOX2 is amplified and overexpressed in some malignant tumors such as squamous cell, lung, prostate, breast, esophageal cell, colon, ovarian, glioblastoma, pancreatic cancer, gastric cancer, head and neck squamous cell carcinoma. To generate a hydroxylated clean electrode surface, ITO electrodes were treated with NH4OH/H2O2/H2O. Later, ITO‐PET electrode surfaces were modified with 3‐glycidoxypropyl trimethoxysilane (3‐GOPS). Then, Anti‐SOX2 was covalently immobilized onto the electrode surfaces. 3‐GOPS concentration, Anti‐SOX2 concentration and incubation time, SOX2 incubation time were optimized. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were utilized in order to follow up the immobilization processes and the optimization steps of the biosensor. To characterize the analytical properties of constructed immunosensor; linear range, repeatability, reproducibility and regeneration studies were investigated. The linear range of the immunosensor was detected as 0.625 pg/mL–62.5 pg/mL. Square wave voltammetry technique was also applied to the biosensor. Storage life of the biosensor was determined for identifying the possible usability of the biosensor in clinical field. Finally, the designed biosensor was applied to the real human serum samples. The results obtained with the presented biosensor were also compared with ELISA results.
中文翻译：
一种用于分析SOX2的高灵敏度，可再现和一次性使用的免疫传感器
在这项研究中，基于ITO（铟锡氧化物）的生物传感器被构造为检测SOX2。SOX2有助于调节细胞的多能性，并与早期胚胎发育，神经和性分化密切相关。SOX2在某些恶性肿瘤（如鳞状细胞癌，肺癌，前列腺癌，乳腺癌，食管细胞癌，结肠癌，卵巢癌，成胶质细胞瘤，胰腺癌，胃癌，头颈部鳞状细胞癌）中扩增并过表达。为了产生羟基化的清洁电极表面，ITO电极用NH 4 OH / H 2 O 2 / H 2处理O.后来，使用3-环氧丙氧基丙基三甲氧基硅烷（3-GOPS）对ITO-PET电极表面进行了改性。然后，将Anti-SOX2共价固定在电极表面上。优化了3-GOPS浓度，抗SOX2浓度和孵育时间，SOX2孵育时间。为了追踪生物传感器的固定过程和优化步骤，利用了电化学阻抗谱（EIS）和循环伏安法（CV）。表征构建的免疫传感器的分析特性；研究了线性范围，重复性，再现性和再生研究。免疫传感器的线性范围为0.625 pg / mL–62.5 pg / mL。方波伏安技术也被应用于生物传感器。确定生物传感器的储存寿命以鉴定生物传感器在临床领域中的可能可用性。最后，将设计的生物传感器应用于真实的人血清样品。还使用本生物传感器获得的结果与ELISA结果进行了比较。
更新日期：2020-01-22
中文翻译：
一种用于分析SOX2的高灵敏度，可再现和一次性使用的免疫传感器
在这项研究中，基于ITO（铟锡氧化物）的生物传感器被构造为检测SOX2。SOX2有助于调节细胞的多能性，并与早期胚胎发育，神经和性分化密切相关。SOX2在某些恶性肿瘤（如鳞状细胞癌，肺癌，前列腺癌，乳腺癌，食管细胞癌，结肠癌，卵巢癌，成胶质细胞瘤，胰腺癌，胃癌，头颈部鳞状细胞癌）中扩增并过表达。为了产生羟基化的清洁电极表面，ITO电极用NH 4 OH / H 2 O 2 / H 2处理O.后来，使用3-环氧丙氧基丙基三甲氧基硅烷（3-GOPS）对ITO-PET电极表面进行了改性。然后，将Anti-SOX2共价固定在电极表面上。优化了3-GOPS浓度，抗SOX2浓度和孵育时间，SOX2孵育时间。为了追踪生物传感器的固定过程和优化步骤，利用了电化学阻抗谱（EIS）和循环伏安法（CV）。表征构建的免疫传感器的分析特性；研究了线性范围，重复性，再现性和再生研究。免疫传感器的线性范围为0.625 pg / mL–62.5 pg / mL。方波伏安技术也被应用于生物传感器。确定生物传感器的储存寿命以鉴定生物传感器在临床领域中的可能可用性。最后，将设计的生物传感器应用于真实的人血清样品。还使用本生物传感器获得的结果与ELISA结果进行了比较。
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