当前位置：
X-MOL 学术
›
J. Agric. Food Chem.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Optimization and Multiomic Basis of Phenyllactic Acid Overproduction by Lactobacillus plantarum.
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b07136 Wenyu Wu 1 , Gang Deng 2 , Chenjian Liu 1 , Xiaowei Gong 3 , Guanghui Ma 4 , Qingqing Yuan 2 , En Yang 1 , Xiaoran Li 1 , Yiyong Luo 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2020-01-22 , DOI: 10.1021/acs.jafc.9b07136 Wenyu Wu 1 , Gang Deng 2 , Chenjian Liu 1 , Xiaowei Gong 3 , Guanghui Ma 4 , Qingqing Yuan 2 , En Yang 1 , Xiaoran Li 1 , Yiyong Luo 1
Affiliation
|
The goal of this study was to explore the regulatory mechanisms of phenyllactic acid (PLA) overaccumulation in Lactobacillus plantarum. The dynamics of PLA production revealed that 24 h was a suitable fermentation time, at which one of the largest differences in PLA content between strains S1 and YM-4-3 was 22.42 mg/L. Additionally, an optimization experiment showed that PLA production under the optimal condition (sample YM-4-3y) was up to 400.74 mg/L, 7.61-13.26 times as those of YM-4-3 and S1. Subsequently, an integrated analysis of genomic, transcriptomic and metabolomic data revealed that, YM-4-3 and YM-4-3y, compared with S1, although lacking a complete de novo biosynthetic pathway, increased PLA production by strengthening the core pathway and central carbon metabolism, and weakening the biosynthesis pathway of amino acids and their derivatives. These changes can provide sufficient precursors and compensate for or balance the energy consumed by the reinforced core pathway.
中文翻译：
植物乳杆菌过量生产苯乳酸的优化和多组学基础。
这项研究的目的是探索植物乳杆菌中苯乳酸（PLA）过度积累的调节机制。PLA生产的动力学表明，24 h是合适的发酵时间，此时菌株S1和YM-4-3之间PLA含量的最大差异之一是22.42 mg / L。此外，优化实验表明，在最佳条件下（样品YM-4-3y），PLA的产量高达400.74 mg / L，是YM-4-3和S1的7.61-13.26倍。随后，对基因组，转录组和代谢组学数据的综合分析显示，与S1相比，YM-4-3和YM-4-3y尽管缺乏完整的从头生物合成途径，但通过加强核心途径和中心途径增加了PLA的产生碳代谢 并削弱氨基酸及其衍生物的生物合成途径。这些变化可以提供足够的前驱物，并补偿或平衡增强型芯通道消耗的能量。
更新日期：2020-01-31
中文翻译：
植物乳杆菌过量生产苯乳酸的优化和多组学基础。
这项研究的目的是探索植物乳杆菌中苯乳酸（PLA）过度积累的调节机制。PLA生产的动力学表明，24 h是合适的发酵时间，此时菌株S1和YM-4-3之间PLA含量的最大差异之一是22.42 mg / L。此外，优化实验表明，在最佳条件下（样品YM-4-3y），PLA的产量高达400.74 mg / L，是YM-4-3和S1的7.61-13.26倍。随后，对基因组，转录组和代谢组学数据的综合分析显示，与S1相比，YM-4-3和YM-4-3y尽管缺乏完整的从头生物合成途径，但通过加强核心途径和中心途径增加了PLA的产生碳代谢 并削弱氨基酸及其衍生物的生物合成途径。这些变化可以提供足够的前驱物，并补偿或平衡增强型芯通道消耗的能量。
京公网安备 11010802027423号