Myocardial infarction occurs because coronary arteries insufficiency is one of the major causes of mortality worldwide. Recent studies have shown that tissue engineering of myocardial tissue to regenerate infarcted tissue or engineering of the coronary artery may help overcome this problem. In the present research, gelatin and single‐walled carbon nanotube were firstly administrated to physico‐chemically and biologically modulate polyurethane nanofibers. Electrospinning, as versatile and effective technique for production of functional nanoscale fiber, was applied. Incorporation of both gelatin and SWNTs reduced mean diameter of nanofibrous scaffolds from 210 to 140 nm, which influenced on initial cell behavior. Possible interaction between gelatin and SWNTs with polyurethane chains was evaluated using FTIR and DSC techniques. Regarding the incorporation of both gelatin and SWNTs, it was found that hydrophilicity of nanofibrous scaffolds dramatically improved. Scaffold degradation profile was adjusted by incorporation of gelatin. Biomimetic mechanical properties of composite scaffolds like normal blood vessel were developed and SWNTs improved the Young modulus and ultimate strength of scaffolds up to 16.47 ± 0.5 and 23.73 ± 0.5 MPa, respectively. However, addition of gelatin increased elongation at break due to its softening effect. The incorporation of the SWNTs led to significant enhancement of electrical conductivity of the scaffolds. Biological evaluation using SEM and MTT assay demonstrated that nanofibrous surface was covered by confluent and dense layer of both myocardial myoblast and endothelial cells after 7 days of culture, which is crucial for cardiovascular tissue engineering. Results verified that the fabricated scaffolds could be effective for cardiovascular tissue engineering.

中文翻译：

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用于心血管组织工程应用的静电纺聚氨酯-明胶-碳纳米管支架的制备、表征和体外评估。

心肌梗塞的发生是因为冠状动脉供血不足是全世界死亡的主要原因之一。最近的研究表明，心肌组织的组织工程再生梗塞组织或冠状动脉工程可能有助于克服这个问题。在目前的研究中，明胶和单壁碳纳米管首先被用于物理化学和生物调节聚氨酯纳米纤维。静电纺丝作为生产功能性纳米级纤维的通用且有效的技术得到了应用。明胶和单壁碳纳米管的掺入将纳米纤维支架的平均直径从 210 纳米降低到 140 纳米，这会影响初始细胞行为。使用 FTIR 和 DSC 技术评估了明胶和 SWNT 与聚氨酯链之间可能的相互作用。关于明胶和单壁碳纳米管的掺入，发现纳米纤维支架的亲水性显着提高。通过掺入明胶调整支架降解曲线。开发了复合支架如正常血管的仿生力学性能，SWNTs 将支架的杨氏模量和极限强度分别提高到 16.47±0.5 和 23.73±0.5 MPa。然而，由于其软化作用，明胶的添加增加了断裂伸长率。SWNT 的掺入导致支架的导电性显着增强。使用 SEM 和 MTT 测定的生物学评估表明，培养 7 天后，纳米纤维表面被心肌成肌细胞和内皮细胞的融合致密层覆盖，这对心血管组织工程至关重要。结果证实所制备的支架可有效用于心血管组织工程。