当前位置：
X-MOL 学术
›
J. Hazard. Mater.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Directional culture of petroleum hydrocarbon degrading bacteria for enhancing crude oil recovery.
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-01-22 , DOI: 10.1016/j.jhazmat.2020.122160 Hailan Li 1 , Ruiqiu Lai 1 , Yulin Jin 2 , Xinxiang Fang 2 , Kai Cui 3 , Shanshan Sun 1 , Yejing Gong 1 , Haonan Li 1 , Zhongzhi Zhang 1 , Guangqing Zhang 4 , Zhiyong Zhang 1
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-01-22 , DOI: 10.1016/j.jhazmat.2020.122160 Hailan Li 1 , Ruiqiu Lai 1 , Yulin Jin 2 , Xinxiang Fang 2 , Kai Cui 3 , Shanshan Sun 1 , Yejing Gong 1 , Haonan Li 1 , Zhongzhi Zhang 1 , Guangqing Zhang 4 , Zhiyong Zhang 1
Affiliation
|
An oxygen-constrained system of crude oil reservoir environment was constructed to stimulate the growth of indigenous microbes, such as petroleum hydrocarbon-degrading bacteria. Addition of nitrogen and phosphorus sources was investigated for the growth of petroleum hydrocarbon-degrading bacteria. The results show that nitrates and phosphates stimulated the growth of the bacteria and promoted the biodegradation of crude oil as the sole carbon source in this process. The minimum surface tension was 29.63 mN/m when the amounts of the nitrogen (NaNO3: [Formula: see text] = 2:1) and phosphorus (KH2PO4: NaH2PO4 = 5:2) sources added were 0.8 wt% and 1.4 wt%, respectively. Furthermore, the dominant petroleum hydrocarbon-degrading bacteria were shifted from Arcobacter in production water to Pseudomonas after the first subculture and then to Bacillus after the sixth subculture. The heteroatom groups in the crude oil were biodegraded simultaneously with normal alkanes and alkyl cyclohexanes. Addition of the nutrients resulted in microbial growth, microbial community shift, and enhanced microbial degradation.
中文翻译：
定向培养石油烃降解细菌以增强原油采收率。
构建了一个氧气受限的原油储层环境系统，以刺激诸如石油烃降解细菌之类的土著微生物的生长。研究了氮和磷源的添加，以降解石油烃降解细菌。结果表明，在该过程中，硝酸盐和磷酸盐刺激了细菌的生长，并促进了原油作为唯一碳源的生物降解。当氮源（NaNO3：[公式：参见文本] = 2：1）和磷源（KH2PO4：NaH2PO4 = 5：2）的添加量分别为0.8 wt％和1.4 wt％时，最小表面张力为29.63 mN / m。 ， 分别。此外，第一次亚培养后，主要的降解石油烃的细菌从生产用水中的芽孢杆菌转移到假单胞菌，然后在第六次亚培养后转移到芽孢杆菌。原油中的杂原子基团与正构烷烃和烷基环己烷同时被生物降解。营养物的添加导致微生物生长，微生物群落转移和增强的微生物降解。
更新日期：2020-01-22
中文翻译：
定向培养石油烃降解细菌以增强原油采收率。
构建了一个氧气受限的原油储层环境系统，以刺激诸如石油烃降解细菌之类的土著微生物的生长。研究了氮和磷源的添加，以降解石油烃降解细菌。结果表明，在该过程中，硝酸盐和磷酸盐刺激了细菌的生长，并促进了原油作为唯一碳源的生物降解。当氮源（NaNO3：[公式：参见文本] = 2：1）和磷源（KH2PO4：NaH2PO4 = 5：2）的添加量分别为0.8 wt％和1.4 wt％时，最小表面张力为29.63 mN / m。 ， 分别。此外，第一次亚培养后，主要的降解石油烃的细菌从生产用水中的芽孢杆菌转移到假单胞菌，然后在第六次亚培养后转移到芽孢杆菌。原油中的杂原子基团与正构烷烃和烷基环己烷同时被生物降解。营养物的添加导致微生物生长，微生物群落转移和增强的微生物降解。
京公网安备 11010802027423号