Fetal bovine serum attenuating perfluorooctanoic acid-inducing toxicity to multiple human cell lines via albumin binding.,Journal of Hazardous Materials

当前位置： X-MOL 学术 › J. Hazard. Mater. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Fetal bovine serum attenuating perfluorooctanoic acid-inducing toxicity to multiple human cell lines via albumin binding.
Journal of Hazardous Materials ( IF 12.2 ) Pub Date : 2020-01-21 , DOI: 10.1016/j.jhazmat.2020.122109
Ruijia Zhang ₁ , Hui Zhang ₂ , Baowei Chen ₃ , Tiangang Luan ₄

Affiliation

Perfluorooctanoic acid (PFOA), as a typical emerging organic pollutant, can interact with serum albumin. However, it remains to characterize the binding of PFOA with serum albumin and to address the role of this interaction in related toxic effects. We aimed to characterize the interaction between PFOA and albumin for understanding the effects of this interaction on the uptake, distribution, and toxicity of PFOA in human cells. The results showed that viable cell count was significantly enhanced by addition of fetabl bovine serum (FBS) into cell culture medium with 300 μM PFOA treatment. PFOA mainly existed as complexed with FBS, at FBS concentration > 10%, which substantially reduced the absorption efficiency of all cell lines to PFOA. The majority of PFOA was accumulated in the cytosolic fraction, followed by nuclei, and mitochondria. Conclusively, our study suggests that the complexation of organic contaminants with proteins might mitigate their toxicity by reducing cellular uptake.

中文翻译：

胎牛血清通过白蛋白结合减弱了对多种人类细胞系的全氟辛酸诱导毒性。

全氟辛酸（PFOA），作为一种典型的新兴有机污染物，可以与血清白蛋白相互作用。然而，表征PFOA与血清白蛋白的结合以及解决这种相互作用在相关毒性作用中的作用尚待定。我们旨在表征PFOA和白蛋白之间的相互作用，以了解这种相互作用对人细胞中PFOA的摄取，分布和毒性的影响。结果表明，通过用300μMPFOA处理向细胞培养基中添加fetabl牛血清（FBS），可显着提高活细胞计数。PFOA主要与FBS形成复合物，FBS浓度> 10％，这大大降低了所有细胞系对PFOA的吸收效率。PFOA的大部分积累在胞质部分，其次是细胞核和线粒体。

更新日期：2020-01-22

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11