The aim of the study is the determination of adsorption mechanism of two proteins characterized by different internal stability (ovalbumin – OVA, lysozyme – LSZ) on the surface of horsetail herb-based engineered biochar (AC) as well as aggregation degree of the selected solid in the presence of biopolymer in the wide pH range (3-11). The engineered biochar was characterized based on the results of nitrogen adsorption/desorption isotherm method, Boehm titration and elemental compound analysis. The adsorption/aggregation mechanisms were determined using spectrophotometric adsorbed amount measurements, zeta potential, potentiometric titration, turbidity measurements and CPS analyses. Mesoporous horsetail herb-based engineered biochar shows the highest adsorption capacity related to OVA and LSZ at protein isoelectric point (i.e. pH 5 for OVA, pH 11 for LSZ). The OVA and LSZ adsorbed amounts is 395.4 and 493.7 mg/g, respectively. Under the same conditions, the OVA and LSZ presence stimulates the AC particle aggregation. As a result, at selected pH values large protein amounts can easily be removed from the aqueous solution using eco-friendly carbonaceous adsorbent.

该研究的目的是确定两种具有不同内部稳定性（卵清蛋白– OVA，溶菌酶– LSZ）的蛋白质在马尾草基工程生物炭（AC）上的吸附机理以及所选固体的聚集度在宽pH范围（3-11）中存在生物聚合物的情况下。根据氮气吸附/解吸等温线法，Boehm滴定法和元素化合物分析的结果对工程生物炭进行了表征。使用分光光度法的吸附量测量，ζ电势，电位滴定，浊度测量和CPS分析确定吸附/聚集机理。以中孔马尾草为基础的工程生物炭在蛋白质等电点（即OVA的pH为5，对于LSZ，pH为11）。OVA和LSZ的吸附量分别为395.4和493.7 mg / g。在相同条件下，OVA和LSZ的存在会刺激AC粒子聚集。结果，在选定的pH值下，可以使用环保的碳质吸附剂轻松地从水溶液中去除大量蛋白质。