The aim of this study was to develop a new colorimetric and fluorometric chemosensor for Sn (II) & Al(III) detection in half aqueous medium. A new probe containing Bodipys, appended on phenolphthalein (PP-B) was synthesized with multi-step reactions. The structure of intermediate products and target compound was characterized with spectroscopic techniques. The absorption and emission properties of the synthesized PP-B probe were investigated in detail and it was deduced that PP-B probe possesses a specifically real-time, fluorometric and colorimetric response to Sn(II) and Al(III) in H₂O/acetone (v/v: 1:1). The selectivity of the probe was checked in the presence of competing metal ions [Al(III), Zn(II), Cu(II), Cd(II), Fe(II), Sn(II), Hg(II), Co(II), Pb(II), Ga(III), Cr(III)]. It was found that there was a marginal difference in fluorescence response of PP-B towards Sn (II) and Al (III) in the presence of competing metal ions. Under the optimized conditions, 1:2 (ligand:metal) stoichiometries were obtained by Job's plot for Sn(II) and Al(III) complexes, respectively. Cell viability study was carried out to check the applicability of probe in human cancer cell lines. Fluorescence images of the probe (PP-B) in human lung cancer cell lines with XTT (sodium 3′-[1-[(phenylamino)-carbony]-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene-sulfonic acid hydrate) test to detect the presence of Sn (II) and Al (III) were acquired by using a confocal laser scanning microscope.

这项研究的目的是开发一种新的比色和荧光化学传感器，用于半水介质中的Sn（II）和Al（III）检测。通过多步反应合成了一种新的含Bodipys的探针，该探针附在酚酞（PP-B）上。用光谱技术表征了中间产物和目标化合物的结构。详细研究了合成的PP-B探针的吸收和发射特性，推论PP-B探针对H _2中的Sn（II）和Al（III）具有特定的实时，荧光和比色响应。O /丙酮（v / v：1：1）。在存在竞争性金属离子的情况下检查探针的选择性[Al（III），Zn（II），Cu（II），Cd（II），Fe（II），Sn（II），Hg（II）， Co（II），Pb（II），Ga（III），Cr（III）]。发现PP-B的荧光反应存在边际差异在竞争性金属离子的存在下向锡（II）和铝（III）迁移。在最佳条件下，分别通过Sn（II）和Al（III）配合物的Job图获得了1：2（配体：金属）化学计量比。进行了细胞活力研究，以检查探针在人癌细胞系中的适用性。XTT（3'-[1-[（[苯基氨基）-碳] -3,4-四唑]-双（4-甲氧基-6-硝基钠）在人肺癌细胞系中探针（PP-B）的荧光图像用共聚焦激光扫描显微镜进行）苯磺酸水合物）测试以检测是否存在Sn（II）和Al（III）。