Abstract Two-dimensional (2D) MXenes have emerged as an attractive platform to construct electrochemical biosensors because of their excellent electronic properties. However, the self-restacking and agglomeration of MXenes lead to unsatisfactory performance. To overcome these drawbacks, herein, the treatment of Ti3C2 MXene through a simultaneous oxidation and alkalization progress was proposed for the synthesis of 3D sodium titanate nanoribbons (M-NTO). Furthermore, conductive poly(3,4-ethyl-enedioxythiophene) (PEDOT) was anchored on 3D M-NTO to enhance its conductivity. The resulting M-NTO-PEDOT composite possessed high specific surface area, fast electron transfer ability, as well as excellent biocompatibility. Subsequently, Au nanoparticles (AuNPs) were electrodeposited on the surface of M-NTO-PEDOT for prostate specific antigen (PSA) antibodies immobilization. Thus, a facile electrochemical label-free immunosensor for sensitive detection of PSA was fabricated on a basis of AuNPs/M-NTO-PEDOT composite. Benefitting from the synergistic effects of M-NTO, PEDOT and AuNPs, the fabricated M-NTO-PEDOT based immunosensor exhibited good analytical performance with a low limit of detection (LOD) of 0.03 pg L−1 (S/N = 3). Additionally, high stability, good selectivity and recovery were achieved in the process of PSA detection in human serum samples.

中文翻译：

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分层 Ti3C2 MXene 衍生的钛酸钠纳米带/PEDOT 用于前列腺特异性抗原的信号放大电化学免疫测定

摘要 二维 (2D) MXenes 因其优异的电子特性而成为构建电化学生物传感器的有吸引力的平台。然而，MXenes 的自重堆积和团聚导致性能不理想。为了克服这些缺点，本文提出了通过同时氧化和碱化处理 Ti3C2 MXene 来合成 3D 钛酸钠纳米带（M-NTO）。此外，将导电聚（3,4-乙撑二氧噻吩）（PEDOT）锚定在 3D M-NTO 上以提高其导电性。所得M-NTO-PEDOT复合材料具有高比表面积、快速电子转移能力以及优异的生物相容性。随后，金纳米粒子 (AuNPs) 被电沉积在 M-NTO-PEDOT 的表面上，用于固定前列腺特异性抗原 (PSA) 抗体。因此，在 AuNPs/M-NTO-PEDOT 复合材料的基础上制造了一种用于敏感检测 PSA 的简便的电化学无标记免疫传感器。受益于 M-NTO、PEDOT 和 AuNPs 的协同效应，制造的基于 M-NTO-PEDOT 的免疫传感器表现出良好的分析性能，检测下限 (LOD) 为 0.03 pg L-1 (S/N = 3)。此外，在人血清样品中的PSA检测过程中实现了高稳定性、良好的选择性和回收率。制造的基于 M-NTO-PEDOT 的免疫传感器表现出良好的分析性能，检测下限 (LOD) 为 0.03 pg L-1 (S/N = 3)。此外，在人血清样品中的PSA检测过程中实现了高稳定性、良好的选择性和回收率。制造的基于 M-NTO-PEDOT 的免疫传感器表现出良好的分析性能，检测下限 (LOD) 为 0.03 pg L-1 (S/N = 3)。此外，在人血清样品中的PSA检测过程中实现了高稳定性、良好的选择性和回收率。