Abstract Distance-based paper microfluidics have been widely used in environmental and biomedical analysis due to simplicity, speed, low cost and suitability for unprivileged regions with untrained personnel. The fluid behavior in the paper microfluidics is controlled by the surface tension, the temperature, the contact angle, the viscosity of the fluid, the average pore radius of the paper, and the channel width. This review discusses the principle and the applications of distance-based paper microfluidics with emphasis on the different detection reactions. The review also summarizes the techniques used in paper microfluidics manufacturing and advantages/disadvantages of each technique. Sections about reagents-free paper microfluidics and their biomedical applications are also presented. The most challenging technical problems associated with the utilization of distance-based paper microfluidics have been discussed with proposed solutions. The review covers the state-of-the-art applications of distance-based paper microfluidics and the future trends in this area.

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基于距离的纸微流体；原理、技术方面和应用

摘要 基于距离的纸质微流体由于简单、速度快、成本低且适用于未经培训人员的贫困地区，已广泛应用于环境和生物医学分析。纸微流体中的流体行为受表面张力、温度、接触角、流体粘度、纸的平均孔半径和通道宽度的控制。这篇综述讨论了基于距离的纸微流体的原理和应用，重点是不同的检测反应。该评论还总结了纸微流体制造中使用的技术以及每种技术的优缺点。还介绍了有关无试剂纸微流体及其生物医学应用的部分。与使用基于距离的纸微流体相关的最具挑战性的技术问题已与建议的解决方案进行了讨论。该评论涵盖了基于距离的纸微流体技术的最新应用以及该领域的未来趋势。