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A lectin with anti-microbial and anti proliferative activities from Lantana camara, a medicinal plant.
Protein Expression and Purification ( IF 1.4 ) Pub Date : 2020-01-21 , DOI: 10.1016/j.pep.2020.105574
Kavita Y Hiremath 1 , Narasimhappagari Jagadeesh 1 , Shivakumar Belur 1 , Supreeth S Kulkarni 1 , Shashikala R Inamdar 1
Affiliation  

BACKGROUND Lectins are known to possess interesting biological properties such as anti microbial, nematicidal, anti tumor and anti viral activities. Lantana camara from verbenaceae family is a medicinal plant known for possessing anti oxidant and anticancer activities. Since anticancer activity is reported in plant lectins, leaves of Lantana camara was used to check the presence of lectin. METHODS AND RESULTS Here we report the purification, characterization and biological properties of a lectin from Lantana camara (LCL) leaves. LCL was purified by ion exchange chromatography on CM-cellulose column followed by affinity chromatography on mucin coupled Sepharose 4B column and gel filtration chromatography on Superdex G75 column. LCL is a glycoprotein with 10% of the carbohydrate and is blood group non specific. SDS-PAGE analysis of affinity purified LCL showed two proteins with apparent molecular weight of 14.49 kDa and 17.4 kDa which were subsequently separated by Gel filtration chromatography on Superdex G75 column. Hapten inhibition studies of LCL revealed its highest affinity for Chitin, Milibiose, α-D-Methyl galactopyranoside and glycoproteins like mucin, asialomucin. LCL showed strong binding to human colon adenocarcinoma HT29 cells with MFI of 242 which was effectively blocked by 68.1 and 62.5% by both mucin and milibiose. LCL showed dose and time dependent growth inhibitory effects on HT29 cells with IC50 of 3.75  μg/ml at 48 h. LCL has potent antibacterial and anti fungal activity. CONCLUSION LCL can be explored for its clinical potential.

中文翻译:

一种来自药用植物马tana丹(Lantana camara)的具有抗微生物和抗增殖活性的凝集素。

背景技术已知凝集素具有令人感兴趣的生物学特性,例如抗微生物,杀线虫,抗肿瘤和抗病毒活性。马鞭草科的马tana丹(Lantana camara)是一种具有抗氧化和抗癌活性的药用植物。由于据报道植物凝集素具有抗癌活性,因此使用马tana丹(Lantana camara)的叶子来检查凝集素的存在。方法和结果在这里,我们报道了马樱丹(Lantana camara,LCL)叶片中的凝集素的纯化,表征和生物学特性。LCL通过在CM-纤维素柱上的离子交换色谱,然后在粘蛋白偶联的Sepharose 4B柱上的亲和色谱和在Superdex G75柱上的凝胶过滤色谱来纯化。LCL是具有10%碳水化合物的糖蛋白,并且是非特异性的血型。亲和纯化的LCL的SDS-PAGE分析显示,两种蛋白质的表观分子量分别为14.49 kDa和17.4 kDa,随后在Superdex G75色谱柱上通过凝胶过滤色谱分离。对LCL的半抗原抑制研究表明,它对几丁质,米利比糖,α-D-甲基半乳糖吡喃糖苷和糖蛋白(如粘蛋白,脱唾液白蛋白)的亲和力最高。LCL显示与人结肠腺癌HT29细胞的强结合​​,MFI为242,粘蛋白和米利波糖都有效阻断了68.1和62.5%的MFI。LCL对HT29细胞具有剂量和时间依赖性的生长抑制作用,48h时IC50为3.75μg/ ml。LCL具有有效的抗菌和抗真菌活性。结论LCL具有临床潜力。4kDa,随后通过Superdex G75柱上的凝胶过滤色谱分离。对LCL的半抗原抑制研究表明,它对几丁质,米利比糖,α-D-甲基半乳糖吡喃糖苷和糖蛋白(如粘蛋白,脱唾液白蛋白)的亲和力最高。LCL显示与人结肠腺癌HT29细胞的强结合​​,MFI为242,粘蛋白和米利波糖都有效阻断了68.1和62.5%的MFI。LCL对HT29细胞具有剂量和时间依赖性的生长抑制作用,48h时IC50为3.75μg/ ml。LCL具有有效的抗菌和抗真菌活性。结论LCL具有临床潜力。4kDa,随后通过Superdex G75柱上的凝胶过滤色谱分离。对LCL的半抗原抑制研究表明,它对几丁质,米利比糖,α-D-甲基半乳糖吡喃糖苷和糖蛋白(如粘蛋白,脱唾液白蛋白)的亲和力最高。LCL显示出与人结肠腺HT29细胞的强结合​​,其MFI为242,其被粘蛋白和milibiose有效地阻断了68.1和62.5%。LCL对HT29细胞具有剂量和时间依赖性的生长抑制作用,48h时IC50为3.75μg/ ml。LCL具有有效的抗菌和抗真菌活性。结论LCL具有临床潜力。脱钙铝蛋白。LCL显示出与人结肠腺HT29细胞的强结合​​,其MFI为242,其被粘蛋白和milibiose有效地阻断了68.1和62.5%。LCL对HT29细胞具有剂量和时间依赖性的生长抑制作用,48h时IC50为3.75μg/ ml。LCL具有有效的抗菌和抗真菌活性。结论LCL具有临床潜力。脱钙铝蛋白。LCL显示与人结肠腺癌HT29细胞的强结合​​,MFI为242,粘蛋白和米利波糖都有效阻断了68.1和62.5%的MFI。LCL对HT29细胞具有剂量和时间依赖性的生长抑制作用,48h时IC50为3.75μg/ ml。LCL具有有效的抗菌和抗真菌活性。结论LCL具有临床潜力。
更新日期:2020-01-22
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