BACKGROUND Biotin has been reported to be a leading cause of interference on several immunoassay platforms using the streptavidin-biotin immobilization system. While biotin interferences have now been well characterized for several assays, only few data are available on their impact on serological markers of infectious viral diseases. METHODS Overall, 10 healthy volunteers (HVs) received a single 100 mg dose of biotin to evaluate its effect on hepatitis B serological markers. Blood samples were taken several times before and after biotin intake. In addition, spiking experiments were applied to investigate biotin's impact on anti-HIV/p24 Ag and anti-HCV antibody levels. Several procedures designed to overcome this interference were evaluated. RESULTS Biotin intake resulted in a false-negative anti-HBs immunological status (<10 mIU/mL) in 40.0% of cases. According to our anti-HBc and anti-HBe results, biotin intake was associated with 90.0% and 80.0% of false positive results, respectively. At the theoretical biotin peak concentration following a 100 mg intake, 50.0% and 66.6% of anti-HIV and anti-HCV results were false negatives, respectively. All the procedures evaluated to overcome the interference were proven effective. CONCLUSION HBV, HCV, and HIV serological markers are likely to be highly sensitive to biotin. Our data confirm that the scope of biotin interference is broader than commonly described.

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生物素干扰：我们是否忽略了对血清标志物的影响？

背景技术据报道，生物素是使用链霉亲和素-生物素固定系统干扰几种免疫测定平台的主要原因。虽然生物素干扰物已经在几种测定方法中得到了很好的表征，但是只有很少的数据可以证明它们对传染性病毒疾病的血清学标志的影响。方法总体而言，有10名健康志愿者（HVs）接受了100 mg剂量的生物素，以评估其对乙型肝炎血清学指标的影响。在摄入生物素之前和之后均采集了几次血样。此外，通过加标实验来研究生物素对抗HIV / p24 Ag和抗HCV抗体水平的影响。对旨在克服这种干扰的几种程序进行了评估。结果摄入生物素会导致抗HBs免疫反应呈假阴性（< 10 mIU / mL）在40.0％的情况下。根据我们的抗HBc和抗HBe结果，摄入生物素的假阳性结果分别为90.0％和80.0％。在摄入100毫克后的理论生物素峰值浓度下，抗HIV和抗HCV结果分别为50.0％和66.6％为假阴性。评估克服干扰的所有程序均被证明是有效的。结论HBV，HCV和HIV血清学标志物可能对生物素高度敏感。我们的数据证实，生物素干扰的范围比通常描述的要广。抗HIV和抗HCV结果的6％分别为假阴性。评估克服干扰的所有程序均被证明是有效的。结论HBV，HCV和HIV血清学标志物可能对生物素高度敏感。我们的数据证实，生物素干扰的范围比通常描述的要广。抗HIV和抗HCV结果的6％分别为假阴性。评估克服干扰的所有程序均被证明是有效的。结论HBV，HCV和HIV血清学标志物可能对生物素高度敏感。我们的数据证实，生物素干扰的范围比通常描述的要广。