Neisseria meningitidis serogroup B (MenB) has recently become the major cause of invasive meningococcal disease in Poland. Therefore, the purpose of this study was to characterize MenB isolates, responsible for invasive meningococcal disease in 2010-2016, by MLST and sequencing of genes encoding proteins used as 4CMenB vaccine antigens. Two methods of coverage estimation were performed: extrapolation of MATS results of Polish meningococci 2010-2011 (exMATS) and gMATS, which combines genotyping and MATS results. Among 662 isolates 20 clonal complexes (CC) were detected, of which the most frequent were CC32, CC41/44 and CC18, accounting for 31.9%, 16.5% and 12.7%, respectively. A total of 111 combinations of PorA variable regions (VR1/VR2) were found, with P1.7,16 (15.0%) and P1.22,14 (13.6%) being prevalent. Vaccine variant VR2:4 was detected in 7.3% of isolates, mainly representing CC41/44 and non-assigned CC. Eighty five fHbp alleles encoding 74 peptide subvariants were revealed. Subvariant 1.1, a component of 4CMenB, was prevalent (24.2%) and found generally in CC32. Typing of the nhba gene revealed 102 alleles encoding 87 peptides. The most frequent was peptide 3 (22.4%), whereas vaccine peptide 2 was detected in 9.8%, mostly among CC41/44. The nadA gene was detected in 34.0% of isolates and the most prevalent was peptide 1 (variant NadA-1; 71.6%), found almost exclusively in CC32 meningococci. Vaccine peptide 8 (variant NadA-2/3) was identified once. Consequently, 292 completed BAST profiles were revealed. Regarding vaccine coverage, 39.7% of isolates had at least one 4CMenB vaccine variant, but according to exMATS and gMATS the coverage was 83.3% and 86.6%, respectively. In conclusion, Polish MenB (2010-2016) was highly diverse according to MLST and gene alleles encoding 4CMenB vaccine antigens. Some correlations between clonal complexes and variants of examined proteins/BAST profiles were revealed and a high coverage of 4CMenB vaccine was estimated.

中文翻译：

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波兰B群脑膜炎球菌（2010-2016）的遗传变异性，包括4CMenB疫苗成分基因。

脑膜炎奈瑟菌B血清群（MenB）最近已成为波兰侵袭性脑膜炎球菌疾病的主要原因。因此，本研究的目的是通过MLST和编码用作4CMenB疫苗抗原的蛋白质的基因测序来表征2010年至2016年造成侵袭性脑膜炎球菌疾病的MenB分离株。进行了两种覆盖率估计方法：波兰2010-2011年脑膜炎双球菌（exMATS）和gMATS的MATS结果外推，结合了基因分型和MATS结果。在662个分离物中，检测到20个克隆复合物（CC），其中最常见的是CC32，CC41 / 44和CC18，分别占31.9％，16.5％和12.7％。总共发现了111个PorA可变区组合（VR1 / VR2），其中P1.7,16（15.0％）和P1.22,14（13.6％）很普遍。在7中检测到疫苗变体VR2：4。3％的分离株，主要代表CC41 / 44和未分配的CC。揭示了编码74个肽亚变体的85个fHbp等位基因。亚变种1.1是4CMenB的一个组成部分，非常流行（24.2％），通常在CC32中发现。nhba基因的分型显示102个等位基因，编码87个肽。最常见的是肽3（22.4％），而疫苗肽2的检出率为9.8％，主要在CC41 / 44中。在34.0％的分离物中检测到了nadA基因，最普遍的是肽1（变异的NadA-1； 71.6％），几乎仅在CC32脑膜炎球菌中发现。疫苗肽8（变异的NadA-2 / 3）被鉴定了一次。因此，揭示了292个完整的BAST配置文件。关于疫苗覆盖率，有39.7％的分离株具有至少一种4CMenB疫苗变体，但根据exMATS和gMATS的覆盖率分别为83.3％和86.6％。结论，根据MLST和编码4CMenB疫苗抗原的基因等位基因，波兰MenB（2010-2016）的差异很大。揭示了克隆复合物和所检查蛋白/ BAST图谱的变体之间的一些相关性，并估计了4CMenB疫苗的高覆盖率。