We report on the characterization of a novel genomic assembly (ARYD3) recovered from formation water (17.6% salinity) and crude oil enrichment amended by isolated soy proteins (0.2%), and incubated for 100 days under anaerobic conditions at 50 °C. Phylogenetic and phylogenomic analysis demonstrated that the ARYD3 is unaffiliated with all currently described bacterial phyla and candidate phyla, as evident by the low AAI (34.7%), shared gene content (19.4%), and 78.9% 16S rRNA gene sequence similarity to Halothiobacillus neapolitanus, its closest cultured relative. Genomic characterization predicts a slow-growing, non-spore forming, and non-motile Gram-negative rod. Adaptation to high salinity is potentially mediated by the production of the compatible solutes cyclic 2,3-diphosphoglycerate (cDPG), α-glucosylglycerate, as well as the uptake of glycine betaine. Metabolically, the genome encodes primarily aminolytic capabilities for a wide range of amino acids and peptides. Interestingly, evidence of propionate degradation to succinate via methyl-malonyl CoA was identified, suggesting possible capability for syntrophic propionate degradation. Analysis of ARYD3 global distribution patterns identified its occurrence in a very small fraction of Earth Microbiome Project datasets examined (318/27,068), where it consistently represented an extremely rare fraction (maximum 0.28%, average 0.004%) of the overall community. We propose the Candidatus name Mcinerneyibacterium aminivorans gen. nov, sp. nov. for ARYD3^T, with the genome serving as the type material for the novel family Mcinerneyibacteriaceae fam. nov., order Mcinerneyibacteriales ord. nov., class Mcinerneyibacteria class nov., and phylum Mcinerneyibacteriota phyl. nov. The type material genome assembly is deposited in GenBank under accession number VSIX00000000.

我们报告了从地层水（盐度为17.6％）中回收的新型基因组装配（ARYD3）和由分离的大豆蛋白（0.2％）修正的原油富集的特征，并在50°C的厌氧条件下孵育了100天。系统发育和系统发育分析表明，ARYD3与当前描述的所有细菌门和候选门均不相关，这表现为低AAI（34.7％），共享基因含量（19.4％）和78.9％的16S rRNA基因序列与那不勒斯嗜盐杆菌的相似性，最亲近的人。基因组学特征可预测生长缓慢，无孢子形成且非运动性的革兰氏阴性棒。对高盐度的适应可能是由相容性溶质环状2,3-二磷酸甘油酸酯（cDPG），α-葡萄糖基甘油酸酯的产生以及甘氨酸甜菜碱的吸收介导的。代谢上，基因组主要编码多种氨基酸和肽的氨解能力。有趣的是，已鉴定出通过甲基-丙二酰辅酶A将丙酸酯降解为琥珀酸酯的证据，表明可能发生共生丙酸酯降解。对ARYD3全球分布模式的分析表明，它仅出现在所检查的地球微生物组项目数据集中的一小部分（318 / 27,068），在该数据集中始终代表着极为罕见的部分（最大0.28％，平均0.004％）。我们建议暂定名称Mcinerneyibacterium aminivorans根。十一月 十一月 对于ARYD3 ^Ť，与基因组用作所述新家族Mcinerneyibacteriaceae FAM的类型材料。11月，订购Mcinerneyibacteriales ord。11月，Mcinerneyibacteriata类和门。十一月 类型材料基因组装配体以登录号VSIX00000000保存在GenBank中。