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Biophysical approaches for exploring lipopeptide-lipid interactions.
Biochimie ( IF 3.3 ) Pub Date : 2020-01-21 , DOI: 10.1016/j.biochi.2020.01.009 Sathishkumar Munusamy 1 , Renaud Conde 2 , Brandt Bertrand 1 , Carlos Munoz-Garay 1
Biochimie ( IF 3.3 ) Pub Date : 2020-01-21 , DOI: 10.1016/j.biochi.2020.01.009 Sathishkumar Munusamy 1 , Renaud Conde 2 , Brandt Bertrand 1 , Carlos Munoz-Garay 1
Affiliation
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In recent years, lipopeptides (LPs) have attracted a lot of attention in the pharmaceutical industry due to their broad-spectrum of antimicrobial activity against a variety of pathogens and their unique mode of action. This class of compounds has enormous potential for application as an alternative to conventional antibiotics and for pest control. Understanding how LPs work from a structural and biophysical standpoint through investigating their interaction with cell membranes is crucial for the rational design of these biomolecules. Various analytical techniques have been developed for studying intramolecular interactions with high resolution. However, these tools have been barely exploited in lipopeptide-lipid interactions studies. These biophysical approaches would give precise insight on these interactions. Here, we reviewed these state-of-the-art analytical techniques. Knowledge at this level is indispensable for understanding LPs activity and particularly their potential specificity, which is relevant information for safe application. Additionally, the principle of each analytical technique is presented and the information acquired is discussed. The key challenges, such as the selection of the membrane model are also been briefly reviewed.
中文翻译：
探索脂肽-脂质相互作用的生物物理方法。
近年来，脂肽（LPs）因其对多种病原体的广谱抗菌活性及其独特的作用方式而在制药行业引起了广泛关注。这类化合物作为传统抗生素的替代品和害虫防治具有巨大的应用潜力。通过研究LP与细胞膜的相互作用，从结构和生物物理的角度理解LP如何工作，对于合理设计这些生物分子至关重要。已经开发了各种分析技术来以高分辨率研究分子内相互作用。然而，这些工具在脂肽-脂质相互作用研究中几乎没有被利用。这些生物物理学方法将为这些相互作用提供精确的见解。在这里，我们回顾了这些最先进的分析技术。这一水平的知识对于理解 LP 的活动，特别是其潜在的特异性是必不可少的，这是安全应用的相关信息。此外，还介绍了每种分析技术的原理并讨论了所获取的信息。还简要回顾了膜模型的选择等关键挑战。
更新日期：2020-01-22
中文翻译：
探索脂肽-脂质相互作用的生物物理方法。
近年来，脂肽（LPs）因其对多种病原体的广谱抗菌活性及其独特的作用方式而在制药行业引起了广泛关注。这类化合物作为传统抗生素的替代品和害虫防治具有巨大的应用潜力。通过研究LP与细胞膜的相互作用，从结构和生物物理的角度理解LP如何工作，对于合理设计这些生物分子至关重要。已经开发了各种分析技术来以高分辨率研究分子内相互作用。然而，这些工具在脂肽-脂质相互作用研究中几乎没有被利用。这些生物物理学方法将为这些相互作用提供精确的见解。在这里，我们回顾了这些最先进的分析技术。这一水平的知识对于理解 LP 的活动，特别是其潜在的特异性是必不可少的，这是安全应用的相关信息。此外，还介绍了每种分析技术的原理并讨论了所获取的信息。还简要回顾了膜模型的选择等关键挑战。
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