The aim of this study was to investigate whether co-culture of human islets with adipose-derived stem cells (ASCs) can improve islet quality and to evaluate which factors play a role in the protective effect of ASCs against islet dysfunction. Islets and ASCs were cultured in three experimental groups for 24 h, 48 h, and 72 h: 1) indirect co-culture of islets with ASC monolayer (Islets/ASCs); 2) islets alone; and 3) ASCs alone. Co-culture with ASCs improved islet viability and function in all culture time-points analyzed. VEGFA, HGF, IL6, IL8, IL10, CCL2, IL1B, and TNF protein levels were increased in supernatants of islet/ASC group compared to islets alone, mainly after 24 h. Moreover, VEGFA, IL6, CCL2, HIF1A, XIAP, CHOP, and NFKBIA genes were differentially expressed in islets from the co-culture condition compared to islets alone. In conclusion, co-culture of islets with ASCs promotes improvements in islet quality.

中文翻译：

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与人类脂肪干细胞共培养后，人类胰岛质量得到改善。

这项研究的目的是调查人类胰岛与脂肪干细胞（ASC）的共培养是否可以改善胰岛质量，并评估哪些因素在ASC抵抗胰岛功能障碍的保护作用中起作用。将胰岛和ASC在三个实验组中培养24小时，48小时和72小时：1）将胰岛与ASC单层间接间接培养（Islets / ASC）；2）单独的胰岛；3）仅ASC。在所有分析的时间点，与ASC的共培养可改善胰岛的活力和功能。与单独使用胰岛相比，胰岛/ ASC组上清中的VEGFA，HGF，IL6，IL8，IL10，CCL2，IL1B和TNF蛋白水平升高，主要是在24小时后。此外，与单独的胰岛相比，共培养条件下的胰岛中差异表达VEGFA，IL6，CCL2，HIF1A，XIAP，CHOP和NFKBIA基因。结论，