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Black Liquor Valorization by Using Marine Protist Thraustochytrium striatum and the Preliminary Metabolic Mechanism Study
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-01-21 , DOI: 10.1021/acssuschemeng.9b05696 Xiang Li 1 , Mi Li 2 , Yunqiao Pu 3 , Arthur J. Ragauskas 2, 3, 4 , Yi Zheng 1
ACS Sustainable Chemistry & Engineering ( IF 7.1 ) Pub Date : 2020-01-21 , DOI: 10.1021/acssuschemeng.9b05696 Xiang Li 1 , Mi Li 2 , Yunqiao Pu 3 , Arthur J. Ragauskas 2, 3, 4 , Yi Zheng 1
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Black liquor that contains various phenolic compounds from lignin solubilization has been the main byproduct of alkaline pretreatment of lignocellulosic biomass in the biorefinery. In this study, black liquor from alkaline pretreatment of corn stover was used as a sole carbon source for the cultivation of a marine protist, Thraustochytrium striatum. It was found that this strain can grow on black liquor and accumulate valuable products (e.g., fatty acids and carotenoids) simultaneously. Under optimal conditions (pH = 7 and NH4Cl = 2 g/L), the cell mass concentration reached 5.2 g/L with total aromatics decreased from 8.18 to 3.09 g/L within 7 day incubation. Fed-batch cultivation was adopted to increase the contents of total fatty acids and carotenoids to 13% and 0.24 mg/g dry cell mass, respectively. Although various compounds such as sugars and organic acids were detected in black liquor and consumed during microbial fermentation as carbon sources, lignin-derived compounds were identified as the major substrates for T. striatum fermentation. Of total aromatics consumed, monomers including p-coumaric acid, ferulic acid, vanillin, and syringaldehyde were observed to be consumed and converted while polymeric fragments were also depolymerized and degraded. Under optimal conditions, around 50% of the total aromatics was consumed. The dynamic changes of compounds in black liquor indicated that diverse metabolic processes were involved in black liquor degradation and utilization by T. striatum. A strong adaptation of T. striatum to a wide range of pH (3–9) was also observed during black liquor fermentation.
中文翻译：
海洋原生质体纹状体对黑酒的平衡作用及其初步代谢机理研究
包含来自木质素增溶的各种酚类化合物的黑液一直是生物精炼厂中木质纤维素生物质碱性预处理的主要副产物。在这项研究中，将玉米秸秆的碱性预处理制得的黑液用作唯一的碳源，用于种植海洋原生生物纹状体。发现该菌株可以在黑液上生长并同时积累有价值的产物（例如脂肪酸和类胡萝卜素）。在最佳条件下（pH = 7和NH 4Cl = 2 g / L），细胞质量浓度达到5.2 g / L，在7天的孵育中，总芳烃从8.18降至3.09 g / L。分批补料培养使总脂肪酸和类胡萝卜素的含量分别增加至干细胞质量的13％和0.24 mg / g。尽管在黑液中检测到各种化合物（例如糖和有机酸），并在微生物发酵过程中将其消耗为碳源，但木质素衍生的化合物被确定为纹状体发酵的主要底物。的总芳烃消耗，单体，包括p观察到-香豆酸，阿魏酸，香兰素和丁香醛被消耗和转化，同时聚合物片段也解聚和降解。在最佳条件下，消耗了约50％的芳香族化合物。黑液中化合物的动态变化表明纹状体的降解和利用涉及多种代谢过程。黑液发酵过程中也观察到纹状体强烈适应广泛的pH（3–9）。
更新日期：2020-01-22
中文翻译：
海洋原生质体纹状体对黑酒的平衡作用及其初步代谢机理研究
包含来自木质素增溶的各种酚类化合物的黑液一直是生物精炼厂中木质纤维素生物质碱性预处理的主要副产物。在这项研究中，将玉米秸秆的碱性预处理制得的黑液用作唯一的碳源，用于种植海洋原生生物纹状体。发现该菌株可以在黑液上生长并同时积累有价值的产物（例如脂肪酸和类胡萝卜素）。在最佳条件下（pH = 7和NH 4Cl = 2 g / L），细胞质量浓度达到5.2 g / L，在7天的孵育中，总芳烃从8.18降至3.09 g / L。分批补料培养使总脂肪酸和类胡萝卜素的含量分别增加至干细胞质量的13％和0.24 mg / g。尽管在黑液中检测到各种化合物（例如糖和有机酸），并在微生物发酵过程中将其消耗为碳源，但木质素衍生的化合物被确定为纹状体发酵的主要底物。的总芳烃消耗，单体，包括p观察到-香豆酸，阿魏酸，香兰素和丁香醛被消耗和转化，同时聚合物片段也解聚和降解。在最佳条件下，消耗了约50％的芳香族化合物。黑液中化合物的动态变化表明纹状体的降解和利用涉及多种代谢过程。黑液发酵过程中也观察到纹状体强烈适应广泛的pH（3–9）。
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