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Glucose metabolism in brown adipose tissue determined by deuterium metabolic imaging in rats.
International Journal of Obesity ( IF 4.2 ) Pub Date : 2020-01-21 , DOI: 10.1038/s41366-020-0533-7 Mette Ji Riis-Vestergaard 1, 2 , Christoffer Laustsen 3 , Christian Østergaard Mariager 3 , Rolf F Schulte 4 , Steen Bønløkke Pedersen 1, 2, 5 , Bjørn Richelsen 1, 2, 5
International Journal of Obesity ( IF 4.2 ) Pub Date : 2020-01-21 , DOI: 10.1038/s41366-020-0533-7 Mette Ji Riis-Vestergaard 1, 2 , Christoffer Laustsen 3 , Christian Østergaard Mariager 3 , Rolf F Schulte 4 , Steen Bønløkke Pedersen 1, 2, 5 , Bjørn Richelsen 1, 2, 5
Affiliation
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BACKGROUND/OBJECTIVES
Brown adipose tissue (BAT) has gained growing interest as a potential target for treatment of obesity. Currently, the most widely used technique/method for in vivo measurements of BAT activity in humans is 18FDG PET/CT. To supplement these investigations novel radiation-free methods are warranted. Deuterium metabolic imaging (DMI) is a novel modality that combines magnetic resonance spectroscopic (MRS) imaging with deuterium-labelled glucose (2H-glucose). This allows for spatio-temporal and metabolic imaging beyond glucose uptake. We aimed to evaluate if DMI could discriminate glucose metabolism in BAT of cold-acclimatised and thermoneutral rats.
SUBJECTS/METHODS
Male Sprague-Dawley rats were housed in a cold environment (9 °C, n = 10) or at thermoneutrality (30 °C, n = 11) for 1 week. For imaging rats were anaesthetized, received a 2H-glucose (1 M, 1.95 g/kg) bolus and DMI was acquired at baseline followed by 20 min time intervals up to 2 h. Furthermore, Dixon MRI was performed for anatomical determination of the interscapular BAT (iBAT) depot along with dynamic contrast enhanced (DCE) MRI to evaluate perfusion.
RESULTS
2H-glucose signal was higher in cold-acclimatised rats compared with thermoneutral rats (p ≤ 0.001) indicating an overall increase in glucose uptake and metabolism. This was in line with a lower fat/water threshold, higher perfusion and increased UCP1 mRNA expression in iBAT (ninefold increment) of cold-acclimatised rats compared with thermoneutral rats.
CONCLUSIONS
We find that DMI can discriminate cold-acclimatised and thermoneutral BAT in rats. This is the first study to evaluate BAT activity by DMI, which may open up for the use of the non-radioactive DMI method for BAT measurements in humans.
中文翻译：
通过大鼠氘代谢成像测定棕色脂肪组织中的葡萄糖代谢。
背景/目的 棕色脂肪组织 (BAT) 作为治疗肥胖症的潜在目标越来越受到关注。目前，用于人体 BAT 活性的体内测量的最广泛使用的技术/方法是 18FDG PET/CT。为了补充这些研究，需要采用新的无辐射方法。氘代谢成像 (DMI) 是一种将磁共振光谱 (MRS) 成像与氘标记的葡萄糖 (2H-葡萄糖) 相结合的新型方式。这允许超越葡萄糖摄取的时空和代谢成像。我们旨在评估 DMI 是否可以区分冷驯化和热中性大鼠 BAT 中的葡萄糖代谢。对象/方法将雄性 Sprague-Dawley 大鼠饲养在寒冷环境 (9 °C, n = 10) 或热中性 (30 °C, n = 11) 中 1 周。对于成像大鼠被麻醉，接受 2 H-葡萄糖（1 M，1.95 g/kg）推注，并在基线时获得 DMI，随后是 20 分钟的时间间隔，最多 2 小时。此外，Dixon MRI 用于解剖确定肩胛间 BAT (iBAT) 库以及动态对比增强 (DCE) MRI 以评估灌注。结果 与热中性大鼠相比，冷驯化大鼠的 2H-葡萄糖信号更高（p ≤ 0.001），表明葡萄糖摄取和代谢总体增加。这与与热中性大鼠相比，冷驯化大鼠的 iBAT（九倍增量）中较低的脂肪/水阈值、较高的灌注和增加的 UCP1 mRNA 表达是一致的。结论 我们发现 DMI 可以区分大鼠的冷驯化和热中性 BAT。这是第一项通过 DMI 评估 BAT 活动的研究，
更新日期：2020-01-21
中文翻译：
通过大鼠氘代谢成像测定棕色脂肪组织中的葡萄糖代谢。
背景/目的 棕色脂肪组织 (BAT) 作为治疗肥胖症的潜在目标越来越受到关注。目前，用于人体 BAT 活性的体内测量的最广泛使用的技术/方法是 18FDG PET/CT。为了补充这些研究，需要采用新的无辐射方法。氘代谢成像 (DMI) 是一种将磁共振光谱 (MRS) 成像与氘标记的葡萄糖 (2H-葡萄糖) 相结合的新型方式。这允许超越葡萄糖摄取的时空和代谢成像。我们旨在评估 DMI 是否可以区分冷驯化和热中性大鼠 BAT 中的葡萄糖代谢。对象/方法将雄性 Sprague-Dawley 大鼠饲养在寒冷环境 (9 °C, n = 10) 或热中性 (30 °C, n = 11) 中 1 周。对于成像大鼠被麻醉，接受 2 H-葡萄糖（1 M，1.95 g/kg）推注，并在基线时获得 DMI，随后是 20 分钟的时间间隔，最多 2 小时。此外，Dixon MRI 用于解剖确定肩胛间 BAT (iBAT) 库以及动态对比增强 (DCE) MRI 以评估灌注。结果 与热中性大鼠相比，冷驯化大鼠的 2H-葡萄糖信号更高（p ≤ 0.001），表明葡萄糖摄取和代谢总体增加。这与与热中性大鼠相比，冷驯化大鼠的 iBAT（九倍增量）中较低的脂肪/水阈值、较高的灌注和增加的 UCP1 mRNA 表达是一致的。结论 我们发现 DMI 可以区分大鼠的冷驯化和热中性 BAT。这是第一项通过 DMI 评估 BAT 活动的研究，
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