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Evaluation of Magnetite Nanoparticle-Based Toxicity on Embryo–Larvae Stages of Zebrafish (Danio rerio)
ACS Applied Nano Materials ( IF 5.3 ) Pub Date : 2020-02-04 , DOI: 10.1021/acsanm.9b02330
Anna Jurewicz 1 , Shaista Ilyas 1 , Jasleen Kaur Uppal 1 , Ivan Ivandic 2 , Sigrun Korsching 2 , Sanjay Mathur 1
Affiliation  

Iron oxide nanoparticles (NPs) are extensively used in various biomedical applications due to their suitability as carriers of diagnostic and therapeutic agents. Herein, we report on the evaluation of concentration-dependent toxicity studies of iron oxide (magnetite) NPs in zebrafish (Danio rerio). To follow the update of iron oxide NPs in the animal model, particles were functionalized by conjugating a fluorescent dye (Congo red) that serves as an efficient probe to track the uptake and accumulations of nanoparticles from the early life stages of zebrafish. As in vivo model organism to study the diffusion an in vivo toxicity, zebrafish embryos and larvae were treated with different concentrations of [email protected]3O4 conjugates (100–800 μg/mL) for 4–96 h postfertilization (hpf). Monitoring of mortality, hatching rate, and whole-embryo cellular death showed that incubation of low concentrations of the NPs did not exhibit adverse developmental toxicity during embryonic and larval stages of zebrafish. Minimal toxic effects were observed at high concentration (800 μg/mL) of [email protected]3O4, causing mortality and delay in hatching cycles. However, [email protected]3O4 exhibited higher toxic effect on zebrafish larvae, suggesting higher bioavailability of the NPs on the tested animal stage. This study provides an investigation on developmental toxicity in zebrafish caused by Fe3O4 NPs and provides innovative insight into [email protected]3O4 NPs serving as an optical probe to study the potential nanotoxicological effects of NPs in in vivo systems.

中文翻译:

斑马鱼(纳米粒子为基础的磁铁矿毒性的上胚幼虫评估阶段斑马鱼

氧化铁纳米颗粒(NPs)由于适合用作诊断和治疗剂的载体,因此广泛用于各种生物医学应用中。在此,我们报告了斑马鱼(Danio rerio)中氧化铁(磁铁矿)NP的浓度依赖性毒性研究的评估。为了跟踪动物模型中氧化铁NP的更新,通过缀合荧光染料(刚果红)对颗粒进行功能化,该荧光染料可作为有效探针来追踪斑马鱼生命早期的纳米颗粒的吸收和积累。作为研究扩散的体内模型生物体内毒性,斑马鱼的胚胎和幼虫用不同浓度的[电子邮件保护] 3 O 4处理。受精(hpf)后4–96 h内使用偶联物(100–800μg/ mL)。死亡率,孵化率和全胚细胞死亡的监测表明,在斑马鱼的胚胎和幼体阶段,低浓度的NP的孵化不会表现出不利的发育毒性。在[电子邮件保护的] 3 O 4高浓度(800μg/ mL)处观察到最小的毒性作用,从而导致死亡率和孵化周期的延迟。但是,[受电子邮件保护] 3 O 4对斑马鱼幼虫具有更高的毒性作用,这表明被测动物阶段NP的生物利用度更高。这项研究提供了对由Fe 3 O 4引起的斑马鱼发育毒性的研究。NPs并提供对[受电子邮件保护的] 3 O 4 NPs的创新见解,可作为光学探针来研究NPs在体内系统中的潜在纳米毒理作用。
更新日期:2020-02-04
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