Bioactive glasses have transformed healthcare due to their versatility. Bioactive borate glass, in particular, has shown remarkable healing properties for both hard and soft tissues. Incorporating dopants into the composition of bioactive glass helps to control mechanical properties, and it increases their usefulness for clinical applications. Using a bioactive borate glass, 13-93B3 (B3), we investigated eleven dopants on the viability and migration potential of adipose stem cells (ASCs), a therapeutic source of cells used in tissue engineering and cell therapy. Our results show that under standard cell culture conditions, only Cu-doped B3 decreased cell viability, while only Y-doped B3 attracted ASCs as it dissolved in cell culture media. Using a transwell invasion assay, priming ASCs with Co, Fe, Ga, I, Sr, or Zn-doped B3 increased their homing capacity. Because there is widespread interest in optimizing and enhancing the homing efficiency of ASCs and other therapeutic cells, we then tested if priming bone marrow mesenchymal stem cells (BMSCs) with dopants also increased their homing capacity. In the case of BMSCs, there was a significant increase in invasion when cells were primed with any of the doped-B3 glasses. This work shows that incorporating dopants into borate glasses can provide a platform for a safe and efficient method that stimulates endogenous cells and healing mechanisms.

中文翻译：

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成人干细胞对掺杂生物活性硼酸盐玻璃的反应。

生物活性玻璃因其多功能性而改变了医疗保健。尤其是具有生物活性的硼酸盐玻璃，对硬组织和软组织均显示出卓越的愈合性能。将掺杂剂掺入生物活性玻璃的成分有助于控制机械性能，并增加其在临床应用中的实用性。我们使用具有生物活性的硼酸盐玻璃13-93B3（B3），研究了11种掺杂剂对脂肪干细胞（ASC）的活力和迁移潜力的影响，脂肪干细胞是组织工程和细胞疗法中使用的细胞治疗来源。我们的结果表明，在标准的细胞培养条件下，只有Cu掺杂的B3降低了细胞活力，而只有Y掺杂的B3溶解在细胞培养基中才吸引ASC。使用Transwell入侵测定法，用Co，Fe，Ga，I，Sr，或掺杂锌的B3可以提高其归巢能力。由于人们对优化和提高ASC和其他治疗性细胞的归巢效率有着广泛的兴趣，因此我们测试了用掺杂剂引发的骨髓间充质干细胞（BMSC）是否也能提高其归巢能力。对于BMSC，当用任何掺杂的B3玻璃灌注细胞时，侵袭的增加显着。这项工作表明，将掺杂剂掺入硼酸盐玻璃中可以为刺激内源性细胞和愈合机制的安全有效方法提供平台。对于BMSC，当用任何掺杂的B3玻璃灌注细胞时，侵袭的增加显着。这项工作表明，将掺杂剂掺入硼酸盐玻璃中可以为刺激内源性细胞和愈合机制的安全有效方法提供平台。对于BMSC，当用任何掺杂的B3玻璃灌注细胞时，侵袭的增加显着。这项工作表明，将掺杂剂掺入硼酸盐玻璃中可以为刺激内源性细胞和愈合机制的安全有效方法提供平台。